Green Synthesis of Silver Nanoparticles and Evaluation of Their Antibacterial Activity against Multidrug-Resistant Bacteria and Wound Healing Efficacy Using a Murine Model

Green nanotechnology has significant applications in various biomedical science fields. In this study, green-synthesized silver nanoparticles, prepared by using and extracts, were characterized using UV-Vis spectroscopy, dynamic light scattering, X-ray diffraction, scanning electron microscopy, and...

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Bibliographic Details
Published in:Antibiotics (Basel) 2020-12, Vol.9 (12), p.902
Main Authors: Lakkim, Vajravathi, Reddy, Madhava C, Pallavali, Roja Rani, Reddy, Kakarla Raghava, Reddy, Ch Venkata, Inamuddin, Bilgrami, Anwar L, Lomada, Dakshayani
Format: Article
Language:English
Subjects:
Animal models
Antibacterial activity
Antibiotics
Antimicrobial activity
Antimicrobial agents
Antioxidants
Azadirachta indica
Bacteria
Bacterial infections
Cancer
Catharanthus roseus
Chemical compounds
Drug resistance
Electron microscopy
green synthesis method
Herbal medicine
Infections
Light diffraction
Light scattering
Multidrug resistance
multidrug-resistant bacteria
Nanoparticles
Nanotechnology
Pharmacology
Photon correlation spectroscopy
Phytochemicals
Plant extracts
Scanning electron microscopy
Silver
silver nanoparticles
Skin
Spectroscopy
Spectrum analysis
Synthesis
Transmission electron microscopy
Ultraviolet radiation
Wound healing
X-ray diffraction
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Summary:Green nanotechnology has significant applications in various biomedical science fields. In this study, green-synthesized silver nanoparticles, prepared by using and extracts, were characterized using UV-Vis spectroscopy, dynamic light scattering, X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. Silver nanoparticles (Ag NPs) synthesized from leaf extracts of and effectively inhibited the growth of multidrug-resistant (MDR) bacteria isolated from patients with septic wound infections. The maximum bacteriolytic activity of the green-synthesized Ag NPs of and against the MDR bacterium was shown by a zone of inhibition of 19 and 16 mm, respectively. Ag NPs exhibited more bacteriolytic activity than Ag NPs in terms of the zone of inhibition. Moreover, these particles were effective in healing wounds in BALB/c mice. Ag NPs of and enhanced wound healing by 94% ± 1% and 87% ± 1%, respectively. Our data suggest that Ag NPs from and ameliorate excision wounds, and wound healing could be due to their effective antimicrobial activity against MDR bacteria. Hence, these Ag NPs could be potential therapeutic agents for the treatment of wounds.
ISSN:2079-6382
2079-6382
DOI:10.3390/antibiotics9120902