Hepatitis E Virus in Manure and Its Removal by Psychrophilic anaerobic Biodigestion in Intensive Production Farms, Santa Catarina, Brazil, 2018-2019

Hepatitis E virus (HEV) is an important enteric agent that can circulate in swine; it is excreted in manure, and of zoonotic interest. The present study investigated, by RT-qPCR, the circulation of HEV in swine manure from different types of pig farms (maternity, nursery, and grow-finish farms) in S...

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Published in:Microorganisms (Basel) 2020-12, Vol.8 (12), p.2045
Main Authors: Souza, Doris Sobral Marques, Tápparo, Deisi Cristine, Rogovski, Paula, Cadamuro, Rafael Dorighello, de Souza, Estêvão Brasiliense, da Silva, Raphael, Degenhardt, Roberto, Lindner, Juliano De Dea, Viancelli, Aline, Michelon, William, Kunz, Airton, Treichel, Helen, Hernández, Marta, Rodríguez-Lázaro, David, Fongaro, Gislaine
Format: Article
Language:English
Subjects:
Anaerobic processes
Animals
Biofertilizers
Biosafety
Communication
Contamination
Digesters
Efficiency
enteric viruses
Farms
Feces
Fertilizers
Genomes
Hepatitis
Hepatitis E virus
Hogs
Inactivation
Infections
Legislation
Load
Manures
Pig manure
pig production circle
Process controls
psychrophilic anaerobic biodigesters
Swine
Swine production
Variance analysis
virus inactivation
Viruses
Zoonoses
zoonotic pathogens
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Summary:Hepatitis E virus (HEV) is an important enteric agent that can circulate in swine; it is excreted in manure, and of zoonotic interest. The present study investigated, by RT-qPCR, the circulation of HEV in swine manure from different types of pig farms (maternity, nursery, and grow-finish farms) in Santa Catarina State, the major pig production area of Brazil, and also evaluated the HEV removal efficiency of psychrophilic anaerobic biodigesters (PABs). While HEV was consistently detected in manure from grow-finish pig farms (>4 log HEV genome copies (GC) L ), the virus was not detected in manure from maternity and nursery farms. These findings suggest a potential high biosafety status during primary-swine production, with a subsequent contamination in grow-finish production. The anaerobic biodigestion process reduced more than 2 log HEV GC in the processed swine manure. However, the virus concentration in final effluent remained high, with an average value of 3.85 log HEV GC L . Consequently, our results demonstrate that PABs can be a robust tool for effective inactivation of HEV, while reinforcing the need for sanitary surveillance and legislation of swine manure-derived biofertilizers, to avoid the spread of zoonotic enteric pathogens such as HEV.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms8122045