Proteomics Analysis Reveals That Caspase-Like and Metacaspase-Like Activities Are Dispensable for Activation of Proteases Involved in Early Response to Biotic Stress in Triticum aestivum L

• Published in: International journal of molecular sciences 2018-12, Vol.19 (12), p.3991
• Main Authors: Balakireva, Anastasia V, Deviatkin, Andrei A, Zgoda, Victor G, Kartashov, Maxim I, Zhemchuzhina, Natalia S, Dzhavakhiya, Vitaly G, Golovin, Andrey V, Zamyatnin, Jr, Andrey A
• Format: Article
• Language: English
• Subjects: Abiotic stress; Apoptosis; Cascades; Caspase; caspase-like; Cell activation; cultivar; Cultivars; degradome; Enzymes; Flowers & plants; Genomes; Goat grass; Immune response; Immune system; Liquid chromatography; Markov chains; Mass spectrometry; Mass spectroscopy; metacaspase; Metallography; papain-like cysteine protease (PLCP); Pathogens; Peptides; Phylogenetics; Plant growth; Protease; Proteinase; Proteins; Proteolysis; Proteolytic enzymes; Proteomics; Puccinia recondita; Spectroscopy; Stagonospora nodorum; subtilase; Triticum aestivum; Wheat; wheat leaf rust
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms19123991

Plants, including L., are constantly attacked by various pathogens which induce immune responses. Immune processes in plants are tightly regulated by proteases from different families within their degradome. In this study, a wheat degradome was characterized. Using profile hidden Markov model (HMMer) algorithm and Pfam database, comprehensive analysis of the genome revealed a large number of proteases (1544 in total) belonging to the five major protease families: serine, cysteine, threonine, aspartic, and metallo-proteases. Mass-spectrometry analysis revealed a 30% difference between degradomes of distinct wheat cultivars (Khakasskaya and Darya), and infection by biotrophic ( Rob. ex Desm f. sp. tritici) or necrotrophic ( ) pathogens induced drastic changes in the presence of proteolytic enzymes. This study shows that an early immune response to biotic stress is associated with the same core of proteases from the C1, C48, C65, M24, M41, S10, S9, S8, and A1 families. Further liquid chromatography-mass spectrometry (LC-MS) analysis of the detected protease-derived peptides revealed that infection by both pathogens enhances overall proteolytic activity in wheat cells and leads to activation of proteolytic cascades. Moreover, sites of proteolysis were identified within the proteases, which probably represent targets of autocatalytic activation, or hydrolysis by another protease within the proteolytic cascades. Although predicted substrates of metacaspase-like and caspase-like proteases were similar in biotrophic and necrotrophic infections, proteolytic activation of proteases was not found to be associated with metacaspase-like and caspase-like activities. These findings indicate that the response of to biotic stress is regulated by unique mechanisms.