Loading…

Upregulation of the MYB2 Transcription Factor Is Associated with Increased Accumulation of Anthocyanin in the Leaves of Dendrobium bigibbum

Orchids with colorful leaves and flowers have significant ornamental value. Here, we used γ-irradiation-based mutagenesis to produce a mutant that developed purple instead of the normal green leaves. RNA sequencing of the mutant plant identified 2513 differentially expressed genes, including 1870 up...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2020-08, Vol.21 (16), p.5653
Main Authors: Lim, Gah-Hyun, Kim, Se Won, Ryu, Jaihyunk, Kang, Si-Yong, Kim, Jin-Baek, Kim, Sang Hoon
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Orchids with colorful leaves and flowers have significant ornamental value. Here, we used γ-irradiation-based mutagenesis to produce a mutant that developed purple instead of the normal green leaves. RNA sequencing of the mutant plant identified 2513 differentially expressed genes, including 1870 up- and 706 downregulated genes. The purple leaf color of mutant leaves was associated with increased expression of genes that encoded key biosynthetic enzymes in the anthocyanin biosynthetic pathway. In addition, the mutant leaves also showed increased expression of several families of transcription factors including the gene. Transient overexpression of in was associated with increased expression of endogenous anthocyanin biosynthesis genes. Interestingly transient overexpression of orthologous genes from other orchids did not upregulate expression of endogenous anthocyanin biosynthesis genes. Together, these results suggest that the purple coloration of leaves is at least associated with increased expression of the gene, and the orthologs from orchids likely function differently, regardless of their high level of similarity.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms21165653