Highly Efficient Autologous HIV-1 Isolation by Coculturing Macrophage With Enriched CD4 + T Cells From HIV-1 Patients

We described a novel HIV autologous isolation method based in coculturing macrophages and CD4 T-cell-enriched fractions from peripheral blood collected from antiretroviral-treated (ART) HIV patients. This method allows the isolation of high viral titers of autologous viruses, over 10 HIV RNA copies/...

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Bibliographic Details
Published in:Frontiers in virology (online) 2022-04, Vol.2
Main Authors: Xufré, Cristina, González, Tanía, Leal, Lorna, Trubey, Charles M, Lifson, Jeffrey D, Gatell, José María, Alcamí, José, Climent, Núria, García, Felipe, Sánchez-Palomino, Sonsoles
Format: Article
Language:English
Subjects:
autologous HIV immunogen
CD4+ T cells
coculture
HIV isolation
HIV therapeutic vaccine
monocyte-derived macrophages (MDM)
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Summary:We described a novel HIV autologous isolation method based in coculturing macrophages and CD4 T-cell-enriched fractions from peripheral blood collected from antiretroviral-treated (ART) HIV patients. This method allows the isolation of high viral titers of autologous viruses, over 10 HIV RNA copies/ml, and reduces the time required to produce necessary amounts for virus for use as antigens presented by monocyte-derived myeloid cells in HIV therapeutic vaccine approaches. By applying these high titer and autologous virus produced in the patient-derived cells, we intended to elicit a boost of the immunological system response in HIV therapeutic vaccines in clinical trials.
ISSN:2673-818X
2673-818X
DOI:10.3389/fviro.2022.869431