Tannylated Calcium Carbonate Materials with Antacid, Anti-Inflammatory, and Antioxidant Effects

• Published in: International journal of molecular sciences 2021-04, Vol.22 (9), p.4614
• Main Authors: Jung, Sung-Yun, Hwang, Heamin, Jo, Han-Saem, Choi, Somang, Kim, Hak-Jun, Kim, Sung-Eun, Park, Kyeongsoon
• Format: Article
• Language: English
• Subjects: Acids; antacid; Antacids - chemistry; Antacids - pharmacology; anti-inflammation; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - pharmacology; Antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Biocompatibility; Biomedical materials; Calcium carbonate; calcium carbonate (CaCO3); Calcium Carbonate - chemistry; Calcium chloride; Calcium ions; Carbon dioxide; Cells, Cultured; Chondrocytes; Chondrocytes - drug effects; Color; Drug Delivery Systems; Fourier transforms; Free radicals; Humans; Hydrocarbons; Hydrogen peroxide; mRNA; Nanoparticles; Physiology; Reactive oxygen species; ROS scavenging activity; Scanning electron microscopy; Tannic acid; tannic acid (TA); Tannins - chemistry
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms22094614

Calcium carbonate (CaCO )-based materials have received notable attention for biomedical applications owing to their safety and beneficial characteristics, such as pH sensitivity, carbon dioxide (CO ) gas generation, and antacid properties. Herein, to additionally incorporate antioxidant and anti-inflammatory functions, we prepared tannylated CaCO (TA-CaCO ) materials using a simple reaction between tannic acid (TA), calcium (Ca ), and carbonate (CO ) ions. TA-CaCO synthesized at a molar ratio of 1:75 (TA:calcium chloride (CaCl )/sodium carbonate (Na CO )) showed 3-6 μm particles, comprising small nanoparticles in a size range of 17-41 nm. The TA-CaCO materials could efficiently neutralize the acid solution and scavenge free radicals. In addition, these materials could significantly reduce the mRNA levels of pro-inflammatory factors and intracellular reactive oxygen species, and protect chondrocytes from toxic hydrogen peroxide conditions. Thus, in addition to their antacid property, the prepared TA-CaCO materials exert excellent antioxidant and anti-inflammatory effects through the introduction of TA molecules. Therefore, TA-CaCO materials can potentially be used to treat inflammatory cells or diseases.