Rosa davurica Inhibited Allergic Mediators by Regulating Calcium and Histamine Signaling Pathways

• Published in: Plants (Basel) 2023-04, Vol.12 (7), p.1572
• Main Authors: Lim, Seojun, Oh, Sarang, Nguyen, Quynh T N, Kim, Myeongju, Zheng, Shengdao, Fang, Minzhe, Yi, Tae-Hoo
• Format: Article
• Language: English
• Subjects: Allergic diseases; Allergies; allergy; Anaphylaxis; Anti-inflammatory agents; Antiviral activity; Asthma; Ca2+ influx; Calcium influx; Calcium ions; Calcium oxide; Calcium signalling; Cyclooxygenase-2; Cytokines; Degranulation; Dermatitis; Gene expression; Histamine; Histidine; Histidine decarboxylase; histidine decarboxylase (HDC); Hypersensitivity; Immunoglobulin E; Inflammation; Interleukins; Kinases; Lipopolysaccharides; Macrophages; MAP kinase; Nitric oxide; Nitric-oxide synthase; Pathogenesis; Phosphorylation; Plant extracts; Prostaglandin endoperoxide synthase; Protein expression; Proteins; Rhinitis; Rosa davurica; Signal transduction; Signaling; Transcription factors; β-hexosaminidase
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants12071572

Pall. exhibits antioxidant, antiviral, and anti-inflammatory properties; however, its pharmacological mechanism in allergy is yet to be understood. This study confirmed the effects of Pall. leaf extract (RLE) on allergy as a new promising material. To evaluate the therapeutic potential of RLE against allergy, we investigated the effects of RLE on the regulatory β-hexosaminidase, histamine, histidine decarboxylase (HDC), Ca influx, nitric oxide (NO), and cytokines induced by lipopolysaccharide (LPS) and DNP-IgE/BSA in Raw 264.7 and RBL-2H3 cells. Furthermore, we examined the effects of RLE on the signaling pathways of mitogen-activated protein kinase (MAPK) and Ca pathways. After stimulating Raw 264.7 cells with LPS, RLE reduced the release of inflammatory mediators, such as NO, cyclooxygenase (COX)-2, inducible nitric oxygen synthase (iNOS), interleukin (IL)-1β, -6, and tumor necrosis factor (TNF)-α. Also, RLE reduced the β-hexosaminidase, histamine, HDC, Ca influx, Ca pathways, and phosphorylation of MAPK in DNP-IgE/BSA-stimulated RBL-2H3 cells. Our studies indicated that RLE is a valuable ingredient for treating allergic diseases by regulating cytokine release from macrophages and mast cell degranulation. Consequently, these results suggested that RLE may serve as a possible alternative promising material for treating allergies.