The Effects of Olive Leaf Extract on The Testis, Sperm Quality and Testicular Germ Cell Apoptosis in Male Rats Exposed to Busulfan

Busulfan (BU) has a destructive effect on the male reproductive system. The goal of this study was to assess the effects of olive leaf extract (OLE) as a source of antioxidants and phenolic compounds, on BU-induced damages in rat testes. In this experimental study, 40 male Wistar rats were randomly...

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Bibliographic Details
Published in:International journal of fertility & sterility 2019-04, Vol.13 (1), p.57-65
Main Authors: Ganjalikhan Hakemi, Sepideh, Sharififar, Fariba, Haghpanah, Tahereh, Babaee, Abdolreza, Eftekhar-Vaghefi, Seyed Hassan
Format: Article
Language:English
Subjects:
Acids
Aluminum
Animals
Antioxidants
Apoptosis
busulfan
Calibration
Cancer
Drug dosages
Enzymes
Experiments
Flavonoids
Leaves
Liver
Male reproductive system
Medical research
Morphology
Motility
olive extract
Original
Reproductive system
Sperm
spermatogenesis
testicular germ cell
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Summary:Busulfan (BU) has a destructive effect on the male reproductive system. The goal of this study was to assess the effects of olive leaf extract (OLE) as a source of antioxidants and phenolic compounds, on BU-induced damages in rat testes. In this experimental study, 40 male Wistar rats were randomly divided into 5 groups. The control group (CTL) received a single intraperitoneal (i.p.) injection of dimethyl sulfoxide (DMSO), followed by oral administration of distilled water for 5 weeks. In BU group, BU (10 mg/kg) was administrated i.p. once. In cotreatment groups, first, received BU (10 mg/kg, a single i.p. injection) then, OLE was administrated orally at different doses of 250 mg/kg (BU+OLE 250), 500 mg/kg (BU+OLE 500) and 750 mg/kg (BU+OLE 750), for 5 weeks. Next, blood and sperm samples were collected. The left testis was removed to investigate testicular parameters and apoptosis by using H and E and TUNEL staining, respectively. All data were analyzed by SPSS software and a P
ISSN:2008-076X
2008-0778
DOI:10.22074/ijfs.2019.5520