Porphyrin-Based Covalent Organic Frameworks with Donor-Acceptor Structure for Enhanced Peroxidase-like Activity as a Colorimetric Biosensing Platform

Hydrogen peroxide (H O ) and glucose play a key role in many cellular signaling pathways. The efficient and accurate in situ detection of H O released from living cells has attracted extensive research interests. Herein, a new porphyrin-based porous covalent organic framework (TAP-COF) was fabricate...

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Bibliographic Details
Published in:Biosensors (Basel) 2023-01, Vol.13 (2), p.188
Main Authors: Wang, Qian, Lv, Liang, Chi, Wenhao, Bai, Yujiao, Gao, Wenqing, Zhu, Peihua, Yu, Jinghua
Format: Article
Language:English
Subjects:
Analysis
Biological markers
Biosensors
Catalytic activity
cells
Colorimetry
Controllability
covalent organic framework
Diffraction
Electron transport
Enzymes
Evaluation
H2O2
Hydrogen peroxide
Morphology
Oxidation
Peroxidase
peroxidase-like activity
porphyrin
Porphyrins
Properties
Scanning electron microscopy
Substrates
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Summary:Hydrogen peroxide (H O ) and glucose play a key role in many cellular signaling pathways. The efficient and accurate in situ detection of H O released from living cells has attracted extensive research interests. Herein, a new porphyrin-based porous covalent organic framework (TAP-COF) was fabricated via one-step condensation of 1,6,7,12-tetrachloroperylene tetracarboxylic acid dianhydride and 5,10,15,20-tetrakis (4-aminophenyl)porphyrin iron(III). The obtained TAP-COF has high surface areas, abundant surface catalytic active sites, and highly effective electron transport due to its precisely controllable donor-acceptor arrangement and 3D porous structure. Then, the new TAP-COF exhibited excellent peroxidase-like catalytic activity, which could effectively catalyze oxidation of the substrate 3,3',5,5'-tetramethylbenzidine by H O to produce a typical blue-colored reaction. On this basis, simple, rapid and selective colorimetric methods for in situ H O detection were developed with the detection limit of 2.6 nM in the wide range of 0.01 to 200 μM. The colorimetric approach also could be used for in situ detection of H O released from living MCF-7 cells. This portable sensor based on a COF nanozyme not only opens a new path for point-of-care testing, but also has potential applications in the field of cell biology and clinical diagnosis.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios13020188