FDCSP Is an Immune-Associated Prognostic Biomarker in HPV-Positive Head and Neck Squamous Carcinoma

• Published in: Biomolecules (Basel, Switzerland) Switzerland), 2022-10, Vol.12 (10), p.1458
• Main Authors: Wu, Qingqing, Shao, Tingru, Huang, Guangzhao, Zheng, Zenan, Jiang, Yingtong, Zeng, Weisen, Lv, Xiaozhi
• Format: Article
• Language: English
• Subjects: Biological markers; Biomarkers; Biomarkers, Tumor - genetics; Biomarkers, Tumor - metabolism; Cancer; Causes of; Chemokines; CIBERSORT; Complications and side effects; CXCL13; CXCL13 protein; Datasets; Dendritic cells; Dendritic Cells, Follicular - metabolism; FDCSP; Gene expression; Genomes; Genomics; Head and neck cancer; Head and neck carcinoma; Head and Neck Neoplasms - genetics; head and neck squamous carcinoma (HNSC) TCGA; Health aspects; Helper cells; HPV; Human papillomavirus; Humans; immune cell infiltration; Immunology; Immunotherapy; Infections; Infiltration; Ligands; Medical prognosis; Mutation; p53 Protein; Papillomavirus infections; Papillomavirus Infections - genetics; Peptides; Prognosis; Proteins; Software; Squamous cell carcinoma; Squamous Cell Carcinoma of Head and Neck - genetics; Transcriptomes
• ISSN: 2218-273X; 2218-273X
• DOI: 10.3390/biom12101458

Head and neck squamous carcinoma (HNSC) poses a major threat to human life. The role of human papillomavirus (HPV) infection in the initiation and progression of HNSC is becoming more widely accepted. HPV-positive (HPV+) HNSC has shown unique responses to cancer therapies, which may be due to differences in immune cell infiltration. It is critical to determine how the immune responses to HPV in HNSC are regulated. Transcriptome data of HNSC from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) database were analyzed. Then, the CIBERSORT algorithm was used to calculate immune cell infiltration in HNSC. FDCSP expression level was detected by qPCR in the HNSC tissues collected from the Nanfang Hospital. Follicular dendritic cell secreted protein (FDCSP) was highly expressed in HPV+ HNSC, and higher expression of FDSCP was associated with a favorable prognosis. In HPV+ HNSC samples, FDCSP significantly increased the proportion of T follicular helper cells (TFHs). FDCSP expression was also found to be associated with TP53 mutation status in HPV+ HNSC. The function of FDCSP was intimately connected to chemokine pathways, particularly with the C-X-C motif chemokine ligand 13 (CXCL13). We verified that the high expression of FDCSP in HPV+ HNSC and higher FDCSP is closely related to prognosis in HNSC samples we collected by qPCR. Collectively, these findings may provide fresh evidence that FDCSP is a potential chemokine-associated prognostic biomarker in HPV+ HNSC.