The root pathogen Aphanomyces euteiches secretes modular proteases in pea apoplast during host infection

To successfully colonize the host, phytopathogens have developed a large repertoire of components to both combat the host plant defense mechanisms and to survive in adverse environmental conditions. Microbial proteases are predicted to be crucial components of these systems. In the present work, we...

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Bibliographic Details
Published in:Frontiers in plant science 2023-03, Vol.14, p.1140101-1140101
Main Authors: Kiselev, Andrei, Camborde, Laurent, Carballo, Laura Ossorio, Kaschani, Farnusch, Kaiser, Markus, van der Hoorn, Renier A L, Gaulin, Elodie
Format: Article
Language:English
Subjects:
Aphanomyces
apoplast
extracellular
Life Sciences
plant pathogen
Plant Science
proteases
root rot
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Summary:To successfully colonize the host, phytopathogens have developed a large repertoire of components to both combat the host plant defense mechanisms and to survive in adverse environmental conditions. Microbial proteases are predicted to be crucial components of these systems. In the present work, we aimed to identify active secreted proteases from the oomycete , which causes root rot diseases on legumes. Genome mining and expression analysis highlighted an overrepresentation of microbial tandemly repeated proteases, which are upregulated during host infection. Activity Based Protein Profiling and mass spectrometry (ABPP-MS) on apoplastic fluids isolated from pea roots infected by the pathogen led to the identification of 35 active extracellular microbial proteases, which represents around 30% of the genes expressed encoding serine and cysteine proteases during infection. Notably, eight of the detected active secreted proteases carry an additional C-terminal domain. This study reveals novel active modular extracellular eukaryotic proteases as potential pathogenicity factors in genus.
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2023.1140101