Intoxication of Mesotrione in Corn Inoculated with Azospirillum brasilense and with Application of Plant Growth Regulators

• Published in: Advances in Weed Science 2019, Vol.37
• Main Authors: BULEGON, L.G., GUIMARÃES, V.F., BATTISTUS, A.G., COSTA, N.V.
• Format: Article
• Language: English
• Subjects: Abiotic stress; Antioxidants; Azospirillum brasilense; Carotenoids; catalase; Cereal crops; Chlorophyll; Corn; Crop yield; Crops; Enzymes; Fluorescence; fluorescence of chlorophyll a; Gas exchange; Gibberellins; Growth regulators; Herbicides; Humidity; Inoculation; Intoxication; peroxidase; Photosynthesis; Plant growth; Plant protection; PLANT SCIENCES; Seeds; Zea mays
• ISSN: 0100-8358; 1806-9681; 1806-9681; 2675-9462
• DOI: 10.1590/s0100-83582019370100058

The objective of this study was to evaluate the effect of intoxication by mesotrione herbicide on the initial development and yield of maize after seed inoculation or foliar spray application with Azospirillum brasilense and application of plant regulators. Therefore, three experiments were performed: one under greenhouse conditions and two under field conditions, in different locations. In all experiments, a randomized block design was used with a 2 x 4 factorial arrangement, representing presence (192 g ha-1) or absence of mesotrione and four treatments corresponding to the control; seed inoculation with A. brasilense; foliar spray application of A. brasilense; foliar spay application of auxin + gibberellin + cytokine (AX + GA + CK), and foliar spay applications with mesotrione were carried out at the V3 stage of the crop. The results from the greenhouse experiment, with mesotrione application, were intoxication of 7.12%, which reduced total chlorophyll content by 10.15% and carotenoid content by 75.86%, leading to reductions in gas exchange and chlorophyll a fluorescence, and increased activity of antioxidative enzymes. Under field conditions, the treatments did not reduce the effects of intoxication during the initial development of maize crop and did not increase crop yield. It was concluded that the use of A. brasilense and plant regulators did not protect maize against mesotrione intoxication in the initial development, nor did it increase yield. RESUMO: Objetivou-se neste estudo avaliar a intoxicação do herbicida Mesotrione no desenvolvimento inicial e na produtividade do milho, inoculado via semente ou foliar com Azospirillum brasilense e aplicação de reguladores vegetais. Portanto, foram realizados três experimentos: um em condição de casa de vegetação e dois em condições de campo, em diferentes locais. Em todos eles utilizou-se o delineamento em blocos casualizados no esquema fatorial 2 x 4, representando presença (192 g ha-1) ou ausência de mesotrione e quatro tratamentos, correspondentes à testemunha, inoculação das sementes com A. brasilense, aplicação foliar de A. brasilense e aplicação foliar de auxina + giberelina + citocina (AX+GA+CK), sendo as aplicações foliares com mesotrione realizadas no estádio V3 da cultura. Em casa de vegetação, a aplicação de mesotrione originou intoxicação de 7,12%, o que reduziu em 10,15% o conteúdo de clorofila total e em 75,86% o teor de carotenoides, resultando em reduções nas trocas gasosas, na fluorescênci