Fabrication of bilayer emulsion by ultrasonic emulsification: Effects of chitosan on the interfacial stability of emulsion

• Published in: Ultrasonics sonochemistry 2023-02, Vol.93, p.106296-106296, Article 106296
• Main Authors: Wang, Tengyu, Wang, Shirang, Zhang, Lijuan, Sun, Jiapeng, Guo, Tianhao, Yu, Guoping, Xia, Xiufang
• Format: Article
• Language: English
• Subjects: beta Carotene; Bilayer emulsion; Chitosan; Chitosan - chemistry; Emulsion stability; Emulsions - chemistry; Hydrophobic and Hydrophilic Interactions; Original; Rice bran protein hydrolysate; Ultrasonic emulsification; Ultrasonics; β-carotene
• ISSN: 1350-4177; 1873-2828
• DOI: 10.1016/j.ultsonch.2023.106296

[Display omitted] •Bilayer emulsion was fabricated by ultrasonic emulsification.•Bilayer emulsion was formed with RBPH-FA and chitosan by electrostatic adsorption.•Chitosan improved the stability of RBPH-FA emulsion at pH 5.0.•Emulsion of chitosan 0.3% was more stable than other samples.•Chitosan assembly in the emulsion can improve β-carotene bioavailability. In this study, the stable system of bilayer emulsion was fabricated by ultrasonic emulsification. The effect of chitosan (CS) addition (0.05 %-0.4 %, w/v) at pH 5.0 on the stability of rice bran protein hydrolysate-ferulic acid (RBPH-FA) monolayer emulsion was investigated. It was found that the addition of CS (0.3 %) could form a stable bilayer emulsion. The droplet size was 3.38 μm and the absolute ζ-potential value was 31.52 mV. The bilayer emulsion had better storage stability, oxidation stability and environmental stabilities than the monolayer emulsion. The results of in vitro simulations revealed the bilayer emulsion was able to deliver the β-carotene to the small intestine digestive stage stably and the bioaccessibility was increased from 22.34 % to 61.36 % compared with the monolayer emulsion. The research confirmed that the bilayer emulsion prepared by ultrasonic emulsification can be used for the delivery of hydrophobic functional component β-carotene.