Mesenchymal stem cells can prevent or promote the progression of colon cancer based on their timing of administration

• Published in: Journal of translational medicine 2023-03, Vol.21 (1), p.227-227, Article 227
• Main Authors: Hu, Weiqian, Wang, Weijun, Jiang, Xin, Wang, Zeyu, Lin, Rong
• Format: Article
• Language: English
• Subjects: Angiogenesis; Animal models; Animals; Antigens; Azoxymethane; Bone marrow; Care and treatment; CD4+T cells; Cell culture; Cell differentiation; Colitis; Colitis-associated colon cancer; Colon - pathology; Colon cancer; Colonic Neoplasms - pathology; Colorectal cancer; Cytokines; Cytokines - metabolism; Development and progression; Dextran; Dextran sulfate; Dextran Sulfate - adverse effects; Disease; Disease Models, Animal; Flow cytometry; Health aspects; Immunofluorescence; Immunomodulatory; Immunoregulation; Inflammation; Inflammatory bowel disease; Injection; Interleukin 12; Interleukin 4; Interleukin-12 - metabolism; Interleukin-4 - metabolism; Lamina propria; Localization; Lymphocytes; Lymphocytes T; Mesenchymal Stem Cell Transplantation; Mesenchymal Stem Cells; Metastases; Mice; Mice, Inbred C57BL; Phenotypes; Risk factors; Stem cells; T-Lymphocytes, Regulatory; Testing; Transforming Growth Factor beta - metabolism; Transforming growth factor-b; Tumor necrosis factor-TNF; Tumors
• ISSN: 1479-5876; 1479-5876
• DOI: 10.1186/s12967-023-04028-3

Mesenchymal stem cell (MSC) therapy has been shown to have some therapeutic effects in rodent models and patients with IBD; however, its role in colon tumor models is controversial. In this study, the potential role and mechanisms of bone marrow-derived MSCs (BM-MSCs) in colitis-associated colon cancer (CAC) were investigated. The CAC mouse model was established with azoxymethane (AOM) and dextran sulfate sodium (DSS). The mice were administered an intraperitoneal injection of MSCs once weekly for different periods. The progression of CAC and the cytokine expression in tissues was assessed. Immunofluorescence staining was used to detect MSCs localization. Levels of immune cells in the spleen and lamina propria of the colon were detected using flow cytometry. A co-culture of MSCs and naïve T cells was performed to determine the effect of MSCs on naïve T cell differentiation. Early administration of MSCs inhibited the occurrence of CAC, while late administration promoted the progression of CAC. The inhibitory effect of early injection in mice was characterized by the expression of inflammatory cytokines in colon tissue was decreased, and induction of T regulatory cells (Tregs) infiltration via TGF-β. The promotive effect of late injection was characterized by a shift of T helper (Th) 1/Th2 immune balance toward a Th2 phenotype through IL-4 secretion. IL-12 can reverse this shift to Th2 accumulation in mice. MSCs can curb the progression of colon cancer by inducing Treg accumulation via TGF-β at the early stage of inflammatory transformation but promote the progression of colon cancer by inducing a shift in Th1/Th2 immune balance to Th2 through IL-4 secretion at the late stage. And the immune balance of Th1/Th2 influenced by MSCs could be reversed by IL-12.