The Effect of Piperidine Nitroxides on the Properties of Metalloproteins in Human Red Blood Cells

Nitroxides are stable, low molecular-weight radicals containing a nitroxide group that has an unpaired electron. The presence of a nitroxide group determines their redox properties. The effect of the piperidine nitroxides, Tempo, Tempol, and Tempamine, on metalloproteins (hemoglobin, superoxide dism...

Full description

Saved in:
Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2023-08, Vol.28 (16), p.6174
Main Authors: Bujak-Pietrek, Stella, Pieniazek, Anna, Gwozdzinski, Krzysztof, Gwozdzinski, Lukasz
Format: Article
Language:English
Subjects:
Acids
Analysis
Antioxidants
Blood
Dehydrogenases
Enzymes
Hemoglobin
Hydrogen peroxide
lactate dehydrogenase
Lipids
Metalloproteins
methemoglobin
Molecular weight
nitroxide
Oxidation
Oxidative stress
red blood cells
Superoxide
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nitroxides are stable, low molecular-weight radicals containing a nitroxide group that has an unpaired electron. The presence of a nitroxide group determines their redox properties. The effect of the piperidine nitroxides, Tempo, Tempol, and Tempamine, on metalloproteins (hemoglobin, superoxide dismutase, catalase) and lactate dehydrogenase in red blood cells was investigated in this research. In addition, the level of lipid peroxidation and the level of protein carbonyl groups were examined as indicators of the effect of oxidative stress. Nitroxides increased superoxide dismutase activity and oxidized hemoglobin to methemoglobin, and also slightly decreased the catalase activity of red blood cells treated with nitroxides. Tempol significantly decreased lactate dehydrogenase activity. All three nitroxides had no effect on membrane lipid peroxidation and protein oxidation. Our results confirm that nitroxides have both antioxidant and prooxidative effects in human red blood cells. The piperidine nitroxides do not initiate the oxidation of proteins and lipids in the membranes of human red blood cells.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules28166174