Hepatitis E virus in liver and bile samples from slaughtered pigs of Brazil

The objective of this study was to detect and identify hepatitis E virus (HEV) strains in liver and bile samples from slaughtered pigs in the state of Paraná, Brazil. Liver and bile samples were collected from 118 asymptomatic adult pigs at a slaughterhouse in a major Brazilian pork production area....

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Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz 2012-11, Vol.107 (7), p.935-939
Main Authors: Gardinali, Noemi Rovaris, Barry, Aline Fernandes, Otonel, Rodrigo Alejandro Arellano, Alfieri, Alice Fernandes, Alfieri, Amauri Alcindo
Format: Article
Language:English
Subjects:
Abattoirs
Animals
Bile - virology
Brazil
hepatitis E virus
Hepatitis E virus - genetics
Hepatitis E virus - isolation & purification
Liver - virology
PARASITOLOGY
Phylogeny
Polymerase Chain Reaction
slaughterhouse
Sus scrofa - virology
swine
TROPICAL MEDICINE
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Summary:The objective of this study was to detect and identify hepatitis E virus (HEV) strains in liver and bile samples from slaughtered pigs in the state of Paraná, Brazil. Liver and bile samples were collected from 118 asymptomatic adult pigs at a slaughterhouse in a major Brazilian pork production area. The samples were assayed using a nested reverse transcription-polymerase chain reaction protocol with primer sets targeting open reading frames (ORF)1 and 2 of the HEV genome. HEV RNA was detected in two (1.7%) liver samples and one (0.84%) bile sample using both primers sets. The HEV strains were classified as genotype 3b on the basis of their nucleotide sequences. These data suggest that healthy pigs may be a source of HEV infection for consumers of pig liver and slaughterhouse workers in Brazil.
ISSN:0074-0276
1678-8060
1678-8060
0074-0276
DOI:10.1590/S0074-02762012000700016