Effects of Co-Inoculating Saccharomyces spp. with Bradyrhizobium japonicum on Atmospheric Nitrogen Fixation in Soybeans ( Glycine max (L.))

• Published in: Plants (Basel) 2023-02, Vol.12 (3), p.681
• Main Authors: Zveushe, Obey Kudakwashe, de Dios, Victor Resco, Zhang, Hengxing, Zeng, Fang, Liu, Siqin, Shen, Songrong, Kang, Qianlin, Zhang, Yazhen, Huang, Miao, Sarfaraz, Ahmed, Prajapati, Matina, Zhou, Lei, Zhang, Wei, Han, Ying, Dong, Faqin
• Format: Article
• Language: English
• Subjects: Acetic acid; Acid production; Agrochemicals; Bacteria; Bioavailability; Bradyrhizobium japonicum; co-inoculation; Crop production; Crop yield; Dry weight; Environmental hazards; Farm buildings; Fertilizers; Fungi; Germination; Indoleacetic acid; Inoculation; Microorganisms; Nitrogen; Nitrogen fixation; nitrogen fixation rate; Nitrogenation; Nodules; Nutrition; Phosphorus; Plant growth; Saccharomyces cerevisiae; Saccharomyces exiguus; Seedlings; Seeds; Soil fertility; Soil improvement; Solubilization; soybean; Soybeans; Sustainable agriculture
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants12030681

Crop production encounters challenges due to the dearth of nitrogen (N) and phosphorus (P), while excessive chemical fertilizer use causes environmental hazards. The use of N-fixing microbes and P-solubilizing microbes (PSMs) can be a sustainable strategy to overcome these problems. Here, we conducted a greenhouse pot experiment following a completely randomized blocked design to elucidate the influence of co-inoculating N-fixing bacteria ( ) and PSMs ( and ) on atmospheric N -fixation, growth, and yield. The results indicate a significant influence of interaction on Indole-3-acetic acid production, P solubilization, seedling germination, and growth. It was also found that atmospheric N -fixation, nodule number per plant, nodule dry weight, straw, and root dry weight per plant at different growth stages were significantly increased under dual inoculation treatments relative to single inoculation or no inoculation treatment. Increased seed yield and N and P accumulation were also noticed under co-inoculation treatments. Soil available N was highest under sole bacterial inoculation and lowest under the control treatment, while soil available P was highest under co-inoculation treatments and lowest under the control treatment. We demonstrated that the co-inoculation of N-fixing bacteria and PSMs enhances P bioavailability and atmospheric N -fixation in soybeans leading to improved soil fertility, raising crop yields, and promoting sustainable agriculture.