Structural analysis and immunomodulatory activity of a homopolysaccharide isolated from Parabacteroides distasonis

A homopolysaccharide fraction (PEP-1A) with desired immunomodulatory activity was isolated from culture broth of Parabacteroides distasonis. Structural characterization uncovered that PEP-1A had a molecular weight of 3.40 × 106 Da and possessed mannose merely. Comprehensive analysis of FT-IR, GC–MS...

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Bibliographic Details
Published in:Arabian journal of chemistry 2022-05, Vol.15 (5), p.103755, Article 103755
Main Authors: Zhu, Yanglu, Cai, Ying, Cao, Xueqin, Li, Pei, Liu, Deliang, Ye, Simin, Xu, Zengmei, Shen, Baochun, Liao, Qiongfeng, Li, Hao, Xie, Zhiyong
Format: Article
Language:English
Subjects:
Exopolysaccharide
Immunomodulatory activity
Parabacteroides distasonis
Structural characterization
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Summary:A homopolysaccharide fraction (PEP-1A) with desired immunomodulatory activity was isolated from culture broth of Parabacteroides distasonis. Structural characterization uncovered that PEP-1A had a molecular weight of 3.40 × 106 Da and possessed mannose merely. Comprehensive analysis of FT-IR, GC–MS and 1D/2D NMR confirmed that the structure of PEP-1A was expected as follows: [Display omitted] Finally, the activity assays on RAW 264.7 macrophages showed that PEP-1A could promote proliferation, increase the production of reactive oxygen species (ROS), enhance the phagocytosis, and promote the secretion of nitric oxide (NO) and inflammatory cytokines including IL-1β, IL-6 and TNF-α. Furthermore, PEP-1A could give play to immunoregulation effect through NF-κB, MAPK and Akt signaling pathways. Collectively, the study of PEP-1A offers a molecular underpinning for the future application of PEP-1A as a potential immunostimulant.
ISSN:1878-5352
1878-5379
DOI:10.1016/j.arabjc.2022.103755