Acetic Acid as an Indirect Sink of CO2 for the Synthesis of Polyhydroxyalkanoates (PHA): Comparison with PHA Production Processes Directly Using CO2 as Feedstock

• Published in: Applied sciences 2018-09, Vol.8 (9), p.1416
• Main Authors: Garcia-Gonzalez, Linsey, De Wever, Heleen
• Format: Article
• Language: English
• Subjects: Acetic acid; autotrophic fermentation; Batch culture; Biodegradable materials; Biomass; Biosynthesis; carbon capture and utilization; Carbon dioxide; Cell growth; Consumption; Cultivation; Cupriavidus necator; Fed-batch culture; Fermentation; Fructose; Gases; Glucose; Mechanical properties; Plastics industry; polyhydroxyalkanoate; Polyhydroxyalkanoates; Productivity; Propionic acid; Raw materials; Renewable resources; Substrates; Valeric acid
• ISSN: 2076-3417; 2076-3417
• DOI: 10.3390/app8091416

White biotechnology is promising to transform CO2 emissions into a valuable commodity chemical such as the biopolymer polyhydroxyalkanaotes (PHA). Our calculations indicated that the indirect conversion of acetic acid from CO2 into PHA is an interesting alternative for the direct production of PHA from CO2 in terms of CO2 fixation, H2 consumption, substrate cost, safety and process performance. An alternative cultivation method using acetic acid as an indirect sink of CO2 was therefore developed and a proof-of-concept provided for the synthesis of both the homopolymer poly(3-hydroxybutyrate) (PHB) and the copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). The aim was to compare key performance parameters with those of existing cultivation methods for direct conversion of CO2 to PHA. Fed-batch cultivations for PHA production were performed using a pH-stat fed-batch feeding strategy in combination with an additional Dissolved Oxygen (DO)-dependent feed. After 118 h of fermentation, 60 g/L cell dry matter (CDM) containing 72% of PHB was obtained, which are the highest result values reported so far. Fed-batch cultivations for PHBV production resulted in 65 g/L CDM and 48 g/L PHBV concentration with a 3HV fraction of 27 mol %. Further research should be oriented towards process optimisation, whole process integration and design, and techno-economic assessment.