Preservative spleen surgery and hyperbaric oxygen therapy Cirurgia conservadora do baço e oxigenoterapia hiperbárica

• Published in: Acta cirúrgica brasileira 2007-01, Vol.22, p.21-28
• Main Authors: Isabel Cristina Andreatta Lemos Paulo, Danilo Nagib Salomão Paulo, Luiz Cálice Cintra, Maria Carmem Silva Santos, Hildegardo Rodrigues, Thiago Antunes Ferrari, Tiago Caetano V. de Azevedo, Alcino Lázaro da Silva
• Format: Article
• Language: English
• Subjects: Adverse effects; Baço; Efeitos adversos; Esplenectomia; Oxygen inhalation therapy; Spleen; Splenectomy; Terapia de inalação de oxigenio
• ISSN: 0102-8650; 1678-2674
• DOI: 10.1590/S0102-86502007000700006

PURPOSE: To assess functional and morphological aspects of spleen auto-implants and of the splenic inferior pole of rats, post-operatively treated or not with hyperbaric oxygen, as well as the survival of these animals, were studied. METHODS: Seventy-eight male Wistar rats, weighing between 192 and 283 g ( 238,3 ± 9,6g), were randomly distributed into three groups: Group1-(n=20), spleen manipulation; group 2-(n=36), spleen auto-implantation; group3-(n= 22), subtotal splenectomy preserving the inferior pole. Each group was subdivided as follows: subgroup a, not submitted to hyperbaric oxygen therapy: 1a(n=10), 2a(n=21), 3a(n= 13); subgroup b, submitted to the therapy: 1b(n=10), 2b(n=15), 3b(n=9). Blood was collected pre-operatively and 11 days after surgery, for the estimation of lipids and immunoglobulins and the counting of platelets and Howell-Jolly corpuscles. The spleen and remains were taken for histological study. RESULTS: The number of surviving animals was significantly higher in groups 1(p