Inhibition Of JNK Phosphorylation By Curcumin Analog C66 Protects LPS-Induced Acute Lung Injury

• Published in: Drug design, development and therapy development and therapy, 2019-12, Vol.13, p.4161-4171
• Main Authors: Xiao, Zhongxiang, Xu, Fengli, Zhu, Xiaona, Bai, Bin, Guo, Lu, Liang, Guang, Shan, Xiaoou, Zhang, Yali, Zhao, Yunjie, Zhang, Bing
• Format: Article
• Language: English
• Subjects: acute lung injury; Acute Lung Injury - drug therapy; Acute Lung Injury - metabolism; Acute Lung Injury - pathology; Alveoli; Analysis; Animals; Anthracenes - administration & dosage; Anthracenes - chemistry; Anthracenes - pharmacology; Anti-inflammatory agents; Bronchus; c-Jun protein; c66; Cell number; Cells, Cultured; Chemical compounds; Curcumin; Curcumin - analogs & derivatives; Curcumin - chemistry; Curcumin - pharmacology; Cyclooxygenase-2; Cytokines; Disease Models, Animal; Dose-Response Relationship, Drug; Drug therapy; Edema; Fluorescent antibody technique; Gene expression; Genes; Genetic research; Health aspects; IL-1β; Immunofluorescence; Inflammation; Inflammation - drug therapy; Inflammation - metabolism; Inflammation - pathology; Injections, Intravenous; Injuries; Interleukin 6; jnk; JNK Mitogen-Activated Protein Kinases - antagonists & inhibitors; JNK Mitogen-Activated Protein Kinases - metabolism; JNK protein; Kinases; lipopolysaccharide; Lipopolysaccharides; Lipopolysaccharides - administration & dosage; Lipopolysaccharides - antagonists & inhibitors; Lipopolysaccharides - pharmacology; Lung diseases; Lungs; Male; Medical research; Mice; Mice, Inbred C57BL; Microscopy; Mitogens; Molecular Structure; Neutrophils; Original Research; Pathogenesis; Pharmacology; Phosphorylation; Phosphorylation - drug effects; Pretreatment; Proteins; Pulmonary edema; Staining; Structure-Activity Relationship; Trachea; Transcription factors; Tumor necrosis factor-α
• ISSN: 1177-8881; 1177-8881
• DOI: 10.2147/dddt.s215712

Acute lung injury (ALI) is characterized by high prevalence and high mortality. Thus far, no effective pharmacological treatment has been made for ALI in clinics. Inflammation is critical to the development of ALI. Curcumin analog C66, having reported as an inhibitor of c-Jun N-terminal kinase (JNK), exhibits anti-inflammatory property both in vitro and in vivo. However, whether C66 is capable of reducing lipopolysaccharide (LPS)-induced ALI through the inhibition of inflammation by targeting JNK remains unknown. Intratracheal injection of LPS was employed to build a mouse ALI model. H&E staining, wet/dry ratio, immunofluorescence staining, inflammatory cell detection, and inflammatory gene expression were used to evaluate lung injury and lung inflammation. In vitro, LPS was used to induce the expression of inflammatory cytokines both in protein and gene levels. The results of our studies showed that the pretreatment with C66 and JNK inhibitor SP600125 was capable of attenuating the LPS-induced ALI by detecting pulmonary edema, pathological changes, total protein concentration, and inflammatory cell number in bronchoalveolar lavage fluid (BALF). Besides, C66 and SP600125 also suppressed LPS-induced inflammatory cytokine expression in BALF, serum, and lung tissue. In vitro, LPS-induced production of TNF-α and IL-6 and gene expression of TNF-α, IL-6, IL-1β, and COX-2 could be inhibited by the pretreatment with C66 and SP600125. It was found that C66 and SP600125 could inhibit LPS-induced phosphorylation of JNK both in vitro and in vivo. In brief, our results suggested that C66 protects LPS-induced ALI through the inhibition of inflammation by targeting the JNK pathway. These findings further confirmed the pivotal role of JNK in ALI and implied that C66 is likely to serve as a potential therapeutic agent for ALI.