Metabolic engineering of Yarrowia lipolytica for scutellarin production

Scutellarin related drugs have superior therapeutic effects on cerebrovascular and cardiovascular diseases. Here, an optimal biosynthetic pathway for scutellarin was constructed in Yarrowia lipolytica platform due to its excellent metabolic potential. By integrating multi-copies of core genes from d...

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Published in:Synthetic and systems biotechnology 2022-09, Vol.7 (3), p.958-964
Main Authors: Wang, Yina, Liu, Xiaonan, Chen, Bihuan, Liu, Wei, Guo, Zhaokuan, Liu, Xiangyu, Zhu, Xiaoxi, Liu, Jiayu, Zhang, Jin, Li, Jing, Zhang, Lei, Gao, Yadi, Zhang, Guanghui, Wang, Yan, Choudhary, M. Iqbal, Yang, Shengchao, Jiang, Huifeng
Format: Article
Language:English
Subjects:
Combinatorial gene overexpression
Metabolic engineering
Original
Scutellarin
Yarrowia lipolytica
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Summary:Scutellarin related drugs have superior therapeutic effects on cerebrovascular and cardiovascular diseases. Here, an optimal biosynthetic pathway for scutellarin was constructed in Yarrowia lipolytica platform due to its excellent metabolic potential. By integrating multi-copies of core genes from different species, the production of scutellarin was increased from 15.11 mg/L to 94.79 mg/L and the ratio of scutellarin to the main by-product was improved about 110-fold in flask condition. Finally, the production of scutellarin was improved 23-fold and reached to 346 mg/L in fed-batch bioreactor, which was the highest reported titer for de novo production of scutellarin in microbes. Our results represent a solid basis for further production of natural products on unconventional yeasts and have a potential of industrial implementation.
ISSN:2405-805X
2405-805X
DOI:10.1016/j.synbio.2022.05.009