Preliminary results of random amplification of polymorphic DNA among Triatominae of the phyllosoma complex (Hemiptera, Reduviidae)

In Mexico, Triatoma longipennis (Usinger), Triatoma picturata (Usinger), and Triatoma pallidipennis (Stal), primary Chagas disease vector species of the phyllosoma complex, were analyzed by randomly amplified polymorphic DNA (RAPD). Sixteen decametric primers resolved individual profiles not identic...

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Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz 2003-12, Vol.98 (8), p.1033-1038
Main Authors: Brenière, Simone F, Taveira, Bruno, Bosseno, Marie-France, Ordoñez, Rosalinda, Lozano-Kasten, Felipe, Magallón-Gastélum, Ezequiel, Ouaissi, Ali, Ramsey, Janine
Format: Article
Language:English
Subjects:
Animals
Female
Genetic Markers
Insect Vectors - classification
Insect Vectors - genetics
Male
Mexico
PARASITOLOGY
phyllosoma complex
Phylogeny
random amplification of polymorphic DNA
Random Amplified Polymorphic DNA Technique
Reduviidae
Triatoma infestans
Triatoma pallidipennis
Triatoma picturata
Triatominae
Triatominae - classification
Triatominae - genetics
TROPICAL MEDICINE
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Summary:In Mexico, Triatoma longipennis (Usinger), Triatoma picturata (Usinger), and Triatoma pallidipennis (Stal), primary Chagas disease vector species of the phyllosoma complex, were analyzed by randomly amplified polymorphic DNA (RAPD). Sixteen decametric primers resolved individual profiles not identical, but partially discriminative between species. Analysis based on pairwise presence/absence comparisons between the three species was performed using three primers and two outgroup species Triatoma infestans (Klug) and Triatoma barberi (Usinger). Fifty-three bands in total were scored, although only two bands were constant among the three phyllosoma complex species. Two other bands were constant only for T. longipennis and T. picturata together, and not present in T. pallidipennis. Neighbor Joining tree and the multiple correspondence analysis discriminated T. pallidipennis clearly from the other two species, although there was overlap between T. longipennis and T. picturata. The results indicate a close relationship between the studied species and support the hypothesis of their recent evolution. The suitability of RAPD to discern populations within the species is discussed.
ISSN:0074-0276
1678-8060
0074-0276
1678-8060
DOI:10.1590/S0074-02762003000800010