Effects of Vernonia amygdalina methanol leaf extract and fractions on Ascaridia galli in experimentally infected birds with regard to its pathological effect

• Published in: Bulletin of the National Research Centre 2022-05, Vol.46 (1), p.1-12, Article 131
• Main Authors: Gasaliyu, Khadijat Abidemi, Ajanusi, Oseka Joseph, Suleiman, Mohammed Musa, Dahiru, Sani, Yusuf, Kuburat Hadiza, Kyari, Stephen, Ogwiji, Matthew, Orakpoghenor, Ochuko
• Format: Article
• Language: English
• Subjects: Acetic acid; Albendazole; Anthelmintic; Antiparasitic agents; Ascaridia galli; Birds; Butanol; Caustic soda; Economic impact; Eggs; Entomology; Ethyl acetate; Flavonoids; Helminthiasis; Histopathology; Humanities and Social Sciences; Infections; Intestine; Leaves; Methanol; multidisciplinary; Parasites; Parasitology; Phytochemicals; Plant extracts; Poultry; Research centers; Science; Science (multidisciplinary); Vernonia amygdalina; Veterinary medicine
• ISSN: 2522-8307; 2522-8307
• DOI: 10.1186/s42269-022-00819-8

Background Helminthiasis, usually a chronic problem in poultry, is the major cause of economic losses in the poultry industry. This study was undertaken with the aim of evaluating the in vitro and in vivo anthelmintic effects of Vernonia amygdalina methanol leaf extract and fractions on Ascaridia galli in experimentally infected birds. Standard protocols for extraction, phytochemical screening, isolation and infection of parasite and histological examination were followed. Result At 50 mg/ml albendazole (ALB) caused the highest inhibition of embryonation (97.3%) followed by crude methanol extract (CME) (94.5%), ethyl acetate fraction (EAF) (81.3%) and butanol fraction (BF) (80.3%). The lowest faecal egg counts (FEC) and highest FEC at 21 days post-treatment were observed in the group treated with albendazole (99.2%) and CME at 800 mg/kg (96.9%). Deparasitization at 200 mg/kg caused by ALB was (93.8%), while at 200, 400 and 800 mg/kg, CME was (77.5%, 87.5% and 91.86%), BF was (75.51%, 79.61% and 85.73%) and EAF was (73.48%, 75.5% and 81.63%), respectively. At post-mortem, broiler chickens infected with A. galli and treated with CME, BF and EAF at 200 mg/kg had desquamation of the villi while at 400 and 800 mg/kg treatments, the intestines were intact. Conclusion The extract and fractions of V. amygdalina were found to possess anthelmintic activity against A. galli due to the inhibition of embryonation of eggs and the reduction in faecal egg count; further research is required to fully ascertain their mechanism of action of the extract.