Role of Smad4 from ocular surface ectoderm in retinal vasculature development

To investigate how signals from lens regulate retinal vascular development and neovascularization. Le-Cre transgenic mouse line was employed to inactivate in the surface ectoderm selectively. Standard histological and whole-mount retina staining were employed to reveal morphological changes of retin...

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Bibliographic Details
Published in:International journal of ophthalmology 2020-02, Vol.13 (2), p.231-238
Main Authors: Li, Jing, Zhang, Jin-Song, Zhao, Jiang-Yue, Han, Guo-Ge
Format: Article
Language:English
Subjects:
Basic Research
retinal vasculature
smad4
surface ectoderm
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Summary:To investigate how signals from lens regulate retinal vascular development and neovascularization. Le-Cre transgenic mouse line was employed to inactivate in the surface ectoderm selectively. Standard histological and whole-mount retina staining were employed to reveal morphological changes of retinal vasculature in defective eye. cDNA microarray and subsequent analyses were conducted to investigate the molecular mechanism underlying the vascular phenotype. Quantitative polymerase chain reaction (qPCR) was carried out to verify the microarrays results. We found that inactivation of specifically on surface ectoderm leads to a variety of retinal vasculature anomalies. Microarray analyses and qPCR revealed that Sema3c, Sema3e, Nrp1, Tie1, Sox7, Sox17, and Sox18 are significantly affected in the knockout retinas at different developmental stages, suggesting that ocular surface ectoderm-derived Smad4 can signal to the retina and regulates various angiogenic signaling in the retina. Our data suggest that the cross-talk between ocular surface ectoderm and retina is important for retinal vasculature development, and regulates various signaling associated with sprouting angiogenesis, vascular remodeling and maturation in the retina of mice.
ISSN:2222-3959
2227-4898
DOI:10.18240/ijo.2020.02.05