The influence of the protein Wnt10b as a marker of bone repair of critical size defects fille with autogenous adipose tissue graft: A study in rabbit calvaria

• Published in: Brazilian journal of oral sciences 2017-12, Vol.16, p.1-11
• Main Authors: Klug, Luiz Gustavo, Storer, Carmen Lucia Mueller, Sebastiani, Aline Monise, Bsrbaresco, Bruna Lorena, Giovanini, Alan Fernando, Deliberador, Tatiana Miranda
• Format: Article
• Language: English
• Subjects: Adipose Tissue. Bone regeneration. Rabbits. Wnt proteins
• ISSN: 1677-3225; 1677-3225
• DOI: 10.20396/bjos.v16i0.8651053

The proteins Wnts are considered a key regulator of the early development of the skeleton. Aim: The aim of this study was to evaluate the presence of the protein Wnt10b as a marker of bone repair in critical size defects surgically created in the calvaria of rabbits treated with fragmented autogenous adipose tissue graft. Methods: A total of 28 rabbits were divided into two groups: the Control group (C) and Adipose Tissue Graft group (ATG). A CSD measuring 15 mm in diameter was created in the calvaria of each animal. In rabbits of the C group, the defect was filled only with blood clot, and in ATG group, the defect was filled with fragmented adipose tissue graft. The two groups were divided into two subgroups (n = 7) for euthanasia 15 and 40 days after surgery. Histological and immunohistochemically analyses were performed to evaluate the neoformed bone and the presence/concentration of Wnt10b protein. The Kruskal-Wallis test was performed to compare the means and standard deviations of the number of Wnt10b + cells/mm2 in both groups in each postoperative period. It was assumed a significance level of 5%. Results: After 40 days, the mean concentration of the protein Wnt10b in ATG group was 26.26 (+-6.97) significant higher (p