Physiological relevance, localization and substrate specificity of the alternative (type II) mitochondrial NADH dehydrogenases of Ogataea parapolymorpha

Mitochondria from harbor a branched electron-transport chain containing a proton-pumping Complex I NADH dehydrogenase and three Type II NADH dehydrogenases (NDH-2). To investigate the physiological role, localization and substrate specificity of these enzymes, the growth of various NADH dehydrogenas...

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Bibliographic Details
Published in:Frontiers in microbiology 2024-12, Vol.15, p.1473869
Main Authors: Juergens, Hannes, Mielgo-Gómez, Álvaro, Godoy-Hernández, Albert, Ter Horst, Jolanda, Nijenhuis, Janine M, McMillan, Duncan G G, Mans, Robert
Format: Article
Language:English
Subjects:
bioenergetics
Microbiology
mitochondria
NADH dehydrogensase
Ogataea parapolymorpha
yeast engineering
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Summary:Mitochondria from harbor a branched electron-transport chain containing a proton-pumping Complex I NADH dehydrogenase and three Type II NADH dehydrogenases (NDH-2). To investigate the physiological role, localization and substrate specificity of these enzymes, the growth of various NADH dehydrogenase knockout mutants was quantitatively characterized in shake-flask and chemostat cultures, followed by oxygen-uptake experiments with isolated mitochondria. NAD(P)H:quinone oxidoreduction of the three NDH-2 were individually assessed. Our findings reveal that the respiratory chain contains an internal NADH-accepting NDH-2 (Ndh2-1/OpNdi1), at least one external NAD(P)H-accepting enzyme, and likely additional mechanisms for respiration-linked oxidation of cytosolic NADH. Metabolic regulation appears to prevent competition between OpNdi1 and Complex I for mitochondrial NADH. With the exception of OpNdi1, the respiratory chain of exhibits metabolic redundancy and tolerates deletion of multiple NADH-dehydrogenase genes without compromising fully respiratory metabolism.
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2024.1473869