New strategy for evaluating pancreatic tissue specimens from endoscopic ultrasound‐guided fine needle aspiration and surgery

Background and Aim Preoperative histological evaluation of pancreatic neoplasms is important for guiding the resection strategy and preventing postoperative adverse events. However, conventional endoscopic methods have technical limitations that reduce the accuracy of the histopathological examinati...

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Bibliographic Details
Published in:JGH open 2021-08, Vol.5 (8), p.953-958
Main Authors: Fukuhara, Seiichiro, Iwasaki, Eisuke, Iwano, Tomohiko, Machida, Yujiro, Tamagawa, Hiroki, Kawasaki, Shintaro, Seino, Takashi, Yokose, Takahiro, Endo, Yutaka, Yoshimura, Kentaro, Kashiwagi, Kazuhiro, Kitago, Minoru, Ogata, Haruhiko, Takeda, Sen, Kanai, Takanori
Format: Article
Language:English
Subjects:
Biopsy
Discriminant analysis
endoscopic ultrasound‐guided fine needle aspiration
Endoscopy
Ions
Mass spectrometry
Medical prognosis
Original
Pancreatic cancer
pancreatic ductal adenocarcinoma
probe electrospray ionization mass spectrometry
Scientific imaging
Solvents
Surgery
Tumors
Ultrasonic imaging
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Summary:Background and Aim Preoperative histological evaluation of pancreatic neoplasms is important for guiding the resection strategy and preventing postoperative adverse events. However, conventional endoscopic methods have technical limitations that reduce the accuracy of the histopathological examination. Probe electrospray ionization mass spectrometry (PESI‐MS) may be a useful technique for rapidly evaluating small specimens. Methods This single‐center prospective study included patients with pancreatic neoplasms between October 2018 and December 2019. Pancreatic ductal adenocarcinoma (PDAC) specimens were obtained via endoscopic ultrasound‐guided fine needle aspiration (EUS‐FNA) and non‐neoplastic tissue was obtained via surgery. Specimens were subjected to PESI‐MS and the mass spectra were analyzed using partial least squares regression‐discriminant analysis. Results The study included 40 patients with 20 nonneoplastic specimens and 19 PDAC specimens (1 case of neuroendocrine carcinoma was omitted). All nonneoplastic specimens were sufficient for PESI‐MS analysis, although only 7 of 19 PDAC specimens were sufficient for PESI‐MS analysis because of poor sample quality or insufficient quantity (
ISSN:2397-9070
2397-9070
DOI:10.1002/jgh3.12617