L-Theanine Regulates the Abundance of Amino Acid Transporters in Mice Duodenum and Jejunum via the mTOR Signaling Pathway

The intestine is a key organ for the absorption of amino acids. L-theanine (LTA) is a structural analog of glutamine and a characteristic non-protein amino acid found in tea ( ) that regulates lipid and protein metabolism. The present study explored the role of LTA in intestinal amino acid absorptio...

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Bibliographic Details
Published in:Nutrients 2022-12, Vol.15 (1), p.142
Main Authors: Liu, Kehong, Peng, Yingqi, Lin, Ling, Gong, Zhihua, Xiao, Wenjun, Li, Yinhua
Format: Article
Language:English
Subjects:
Absorption
Alkaline phosphatase
Amino acid metabolism
amino acid transporters
Amino acids
Bioengineering
Data analysis
Dietary supplements
Duodenum
Gene expression
Glutamine
Growth factors
Health aspects
Intestinal absorption
Intestine
Jejunum
Kinases
L-theanine
Laboratory animals
Lipid metabolism
Lipids
Metabolism
mRNA
mTOR signaling pathway
Nitrogen
Physiological aspects
Protein biosynthesis
Protein metabolism
Protein synthesis
Protein turnover
Proteins
S6K1
Signal transduction
Small intestine
Theanine
TOR protein
Urea
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Summary:The intestine is a key organ for the absorption of amino acids. L-theanine (LTA) is a structural analog of glutamine and a characteristic non-protein amino acid found in tea ( ) that regulates lipid and protein metabolism. The present study explored the role of LTA in intestinal amino acid absorption, protein synthesis, and its mechanisms. Overall, our findings suggest that LTA supplementation not only affects serum alkaline phosphatase (AKP), total protein (TP), and urea nitrogen (BUN) levels, but it also upregulates the mRNA and protein expression of amino acid transporters (EAAT3, EAAT1, 4F2hc, y LAT1, CAT1, ASCT2, and B AT1), and activates the mTOR signaling pathway. The downstream S6 and S6K1 proteins are regulated, and the expression of amino acid transporters is regulated. These findings suggest that LTA increases intestinal AA absorption, promotes protein metabolism, and increases nitrogen utilization by upregulating AAT expression, activating the mTOR signaling pathway, and phosphorylating the mTOR downstream proteins S6 and S6K1.
ISSN:2072-6643
2072-6643
DOI:10.3390/nu15010142