Lipopolysaccharide immune stimulation but not β-mannanase supplementation affects maintenance energy requirements in young weaned pigs

• Published in: Journal of animal science and biotechnology 2018-06, Vol.9 (1), p.47-47, Article 47
• Main Authors: Huntley, Nichole F, Nyachoti, C Martin, Patience, John F
• Format: Article
• Language: English
• Subjects: Acute phase proteins; Amino acids; Blood; Calorimetry; Corn; Cytokines; Deposition; Diet; Dietary supplements; Digestibility; E coli; Efficiency; Energy; Energy balance; Energy requirements; Enzymes; Experiments; Feed induced immune response; Feeds; Fever; Haptoglobin; Heat production; Hogs; Immune response; Immune status; Immune system; Inflammation; Insulin; Leukocytes; Lipopolysaccharides; Mannan; Mannanases; Metabolism; Nitrogen balance; Nutrient balance; Nutrient concentrations; Nutrient status; Nutrients; Nutrition research; Partitioning; Poultry; Rodents; Soybeans; Stimulation; Weaning; β-Mannan
• ISSN: 1674-9782; 2049-1891; 2049-1891
• DOI: 10.1186/s40104-018-0264-y

Pathogen or diet-induced immune activation can partition energy and nutrients away from growth, but clear relationships between immune responses and the direction and magnitude of energy partitioning responses have yet to be elucidated. The objectives were to determine how β-mannanase supplementation and lipopolysaccharide (LPS) immune stimulation affect maintenance energy requirements (MEm) and to characterize immune parameters, digestibility, growth performance, and energy balance. In a randomized complete block design, 30 young weaned pigs were assigned to either the control treatment (CON; basal corn, soybean meal and soybean hulls diet), the enzyme treatment (ENZ; basal diet + 0.056% β-mannanase), or the immune system stimulation treatment (ISS; basal diet + 0.056% β-mannanase, challenged with repeated increasing doses of LPS). The experiment consisted of a 10-d adaptation period, 5-d digestibility and nitrogen balance measurement, 22 h of heat production (HP) measurements, and 12 h of fasting HP measurements in indirect calorimetry chambers. The immune challenge consisted of 4 injections of either LPS (ISS) or sterile saline (CON and ENZ), one every 48 h beginning on d 10. Blood was collected pre- and post-challenge for complete blood counts with differential, haptoglobin and mannan binding lectin, 12 cytokines, and glucose and insulin concentrations. Beta-mannanase supplementation did not affect immune status, nutrient digestibility, growth performance, energy balance, or ME . The ISS treatment induced fever, elevated proinflammatory cytokines and decreased leukocyte concentrations (   0.10), but increased total HP (21%) and ME (23%), resulting in decreased lipid deposition (-30%) and average daily gain (-18%) (