Genome-Wide Distribution of Novel Ta-3A1 Mini-Satellite Repeats and Its Use for Chromosome Identification in Wheat and Related Species

A large proportion of the genomes of grasses is comprised of tandem repeats (TRs), which include satellite DNA. A mini-satellite DNA sequence with a length of 44 bp, named Ta-3A1, was found to be highly accumulated in wheat genome, as revealed by a comprehensive sequence analysis. The physical distr...

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Bibliographic Details
Published in:Agronomy (Basel) 2019-01, Vol.9 (2), p.60
Main Authors: Lang, Tao, Li, Guangrong, Yu, Zhihui, Ma, Jiwei, Chen, Qiheng, Yang, Ennian, Yang, Zujun
Format: Article
Language:English
Subjects:
Chromosome rearrangements
Chromosomes
Copy number
Deoxyribonucleic acid
DNA
Evolution
Flowers & plants
Fluorescence
Fluorescence in situ hybridization
Genomes
Germplasm
Goat grass
Hybridization
Labeling
mini-satellite
Nucleotide sequence
Oligonucleotides
Phylogenetics
Polymorphism
Polyploidy
Satellite DNA
Satellites
Sequence analysis
Speciation
tandem repeats
Wheat
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Summary:A large proportion of the genomes of grasses is comprised of tandem repeats (TRs), which include satellite DNA. A mini-satellite DNA sequence with a length of 44 bp, named Ta-3A1, was found to be highly accumulated in wheat genome, as revealed by a comprehensive sequence analysis. The physical distribution of Ta-3A1 in chromosomes 3A, 5A, 5B, 5D, and 7A of wheat was confirmed by nondenaturing fluorescence in situ hybridization (ND-FISH) after labeling the oligonucleotide probe. The analysis of monomer variants indicated that rapid sequence amplification of Ta-3A1 occurred first on chromosomes of linkage group 5, then groups 3 and 7. Comparative ND-FISH analysis suggested that rapid changes occurred in copy number and chromosomal locations of Ta-3A1 among the different species in the tribe Triticeae, which may have been associated with chromosomal rearrangements during speciation and polyploidization. The labeling and subsequent use of Ta-3A1 by ND-FISH may assist in the precise identification and documentation of novel wheat germplasm engineered by chromosome manipulation.
ISSN:2073-4395
2073-4395
DOI:10.3390/agronomy9020060