The Induction of Combined Hyperthermal Ablation Effect of Irreversible Electroporation with Polydopamine Nanoparticle-Coated Electrodes

• Published in: International journal of molecular sciences 2024-04, Vol.25 (8), p.4317
• Main Authors: Jeon, Sung-Min, Davaa, Enkhzaya, Jenjob, Ratchapol, Pechyen, Chiravoot, Natphopsuk, Sitakan, Jeong, Seok, Yoo, Hye Jin, Yang, Su-Geun
• Format: Article
• Language: English
• Subjects: Ablation; Ablation (Surgery); Animals; cancer ablation; Cancer therapies; Cell death; Dopamine; Electrodes; Electroporation - methods; Heat; Hydrogels; hyperthermal effect; Hyperthermia, Induced - methods; Hypotheses; Indoles - chemistry; Indoles - pharmacology; irreversible electroporation (IRE); Liver - drug effects; Liver - surgery; Nanoparticles; Nanoparticles - chemistry; polydopamine nanoparticles; Polymers - chemistry; Protective coatings; Rabbits; Temperature; Thermal energy; Tumors
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25084317

Irreversible electroporation (IRE) is a prominent non-thermal ablation method widely employed in clinical settings for the focal ablation therapy of solid tumors. Utilizing high-voltage, short-duration electric pulses, IRE induces perforation defects in the cell membrane, leading to apoptotic cell death. Despite the promise of irreversible electroporation (IRE) in clinical applications, it faces challenges concerning the coverage of target tissues for ablation, particularly when compared to other thermal ablation therapies such as radiofrequency ablation, microwave ablation, and cryoablation. This study aims to investigate the induced hyperthermal effect of IRE by applying a polydopamine nanoparticle (Dopa NP) coating on the electrode. We hypothesize that the induced hyperthermal effect enhances the therapeutic efficacy of IRE for cancer ablation. First, we observed the hyperthermal effect of IRE using Dopa NP-coated electrodes in hydrogel phantom models and then moved to in vivo models. In particular, in in vivo animal studies, the IRE treatment of rabbit hepatic lobes with Dopa NP-coated electrodes exhibited a two-fold higher increase in temperature (ΔT) compared to non-coated electrodes. Through a comprehensive analysis, we found that IRE treatment with Dopa NP-coated electrodes displayed the typical histological signatures of hyperthermal ablation, including the disruption of the hepatic cord and lobular structure, as well as the infiltration of erythrocytes. These findings unequivocally highlight the combined efficacy of IRE with Dopa NPs for electroporation and the hyperthermal ablation of target cancer tissues.