Extraction of Peptidoglycan from L. paracasei subp. Paracasei X12 and Its Preliminary Mechanisms of Inducing Immunogenic Cell Death in HT-29 Cells

L. paracasei subp. paracasei X12 was previously isolated from a Chinese traditional fermented cheese with anticancer activities and probiotic potential. Herein, the integral peptidoglycan (X12-PG) was extracted by a modified trichloroacetic acid (TCA) method. X12-PG contained the four representative...

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Bibliographic Details
Published in:International journal of molecular sciences 2015-08, Vol.16 (8), p.20033-20049
Main Authors: Tian, Pei-Jun, Li, Bao-Long, Shan, Yu-Juan, Zhang, Jin-Na, Chen, Jing-Yu, Yu, Min, Zhang, Lan-Wei
Format: Article
Language:English
Subjects:
Antineoplastic Agents - isolation & purification
Antineoplastic Agents - pharmacology
Apoptosis
Calcium
Calcium - metabolism
calreticulin
Calreticulin - metabolism
Cell Proliferation - drug effects
Cell Survival - drug effects
Cheese
Colonic Neoplasms - metabolism
Endoplasmic Reticulum Stress - drug effects
Fermentation
Gene Expression Regulation, Neoplastic - drug effects
HMGB1 Protein - metabolism
HT29 Cells
Humans
immunogenic cell death
Lactobacillus - chemistry
peptidoglycan
Peptidoglycan - isolation & purification
Peptidoglycan - pharmacology
Probiotics
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Summary:L. paracasei subp. paracasei X12 was previously isolated from a Chinese traditional fermented cheese with anticancer activities and probiotic potential. Herein, the integral peptidoglycan (X12-PG) was extracted by a modified trichloroacetic acid (TCA) method. X12-PG contained the four representative amino acids Asp, Glu, Ala and Lys, and displayed the similar lysozyme sensitivity, UV-visible scanning spectrum and molecular weight as the peptidoglycan standard. X12-PG could induce the production of apoptotic bodies observed by transmission electron microscopy (TEM). X12-PG could significantly induced the translocation of calreticulin (CRT) and the release of high mobility group box 1 protein (HMGB1), the two notable hallmarks of immunogenic cell death (ICD), with the endoplastic reticulum (ER) damaged and subsequently intracellular [Ca(2+)] elevated. Our findings implied that X12-PG could induce the ICD of HT-29 cells through targeting at the ER. The present results may enlighten the prospect of probiotics in the prevention of colon cancer.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms160820033