Two User-Friendly Molecular Markers Developed for the Identification of Hybrid Lethality Genes in Brassica oleracea

Cabbage (Brassica oleracea) is an important vegetable crop that is cultivated worldwide. Previously, we reported the identification of two dominant complementary hybrid lethality (HL) genes in cabbage that could result in the death of hybrids. To avoid such losses in the breeding process, we attempt...

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Bibliographic Details
Published in:Agronomy (Basel) 2021-05, Vol.11 (5), p.982
Main Authors: Xiao, Zhiliang, Kong, Congcong, Han, Fengqing, Yang, Limei, Zhuang, Mu, Zhang, Yangyong, Wang, Yong, Ji, Jialei, Li, Zhansheng, Fang, Zhiyuan, Lv, Honghao
Format: Article
Language:English
Subjects:
Accuracy
Brassica
Brassica oleracea
breeding
Fertility
Gene polymorphism
Genes
Genomics
Genotypes
hybrid lethality
Hybrids
Identification
Inbreeding
InDel marker
KASP marker
Lethality
Mapping
Markers
Nucleotides
Phenotypes
Plant breeding
Polymorphism
Single-nucleotide polymorphism
Vegetables
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Summary:Cabbage (Brassica oleracea) is an important vegetable crop that is cultivated worldwide. Previously, we reported the identification of two dominant complementary hybrid lethality (HL) genes in cabbage that could result in the death of hybrids. To avoid such losses in the breeding process, we attempted to develop molecular markers to identify HL lines. Among 54 previous mapping markers closely linked to BoHL1 or BoHL2, only six markers for BoHL2 were available in eight cabbage lines (two BoHL1 lines; three BoHL2 lines; three lines without BoHL); however, they were neither universal nor user-friendly in more inbred lines. To develop more accurate markers, these cabbage lines were resequenced at an ~20× depth to obtain more nucleotide variations in the mapping regions. Then, an InDel in BoHL1 and a single-nucleotide polymorphism (SNP) in BoHL2 were identified, and the corresponding InDel marker MBoHL1 and the competitive allele-specific PCR (KASP) marker KBoHL2 were developed and showed 100% accuracy in eight inbred lines. Moreover, we identified 138 cabbage lines using the two markers, among which one inbred line carried BoHL1 and 11 inbred lines carried BoHL2. All of the lethal line genotypes obtained with the two markers matched the phenotype. Two markers were highly reliable for the rapid identification of HL genes in cabbage.
ISSN:2073-4395
2073-4395
DOI:10.3390/agronomy11050982