Voluntary exercise sensitizes cancer immunotherapy via the collagen inhibition-orchestrated inflammatory tumor immune microenvironment

• Published in: Cell reports (Cambridge) 2024-09, Vol.43 (9), p.114697, Article 114697
• Main Authors: Luo, Zhiwen, Mei, Jie, Wang, Xianwen, Wang, Ruixin, He, Zhao, Geffen, Yifat, Sun, Xiaomeng, Zhang, Xingyu, Xu, Junying, Wan, Renwen, Feng, Xinting, Jiao, Chunmeng, Su, Xiaoping, Sun, Junming, Chen, Shiyi, Chen, Jiwu, Mao, Wenjun, Yang, Yunlong, Sun, Yaying
• Format: Article
• Language: English
• Subjects: CP: Cancer; CP: Immunology; extracellular matrix; immunotherapy; microRNA; tumor immune microenvironment; voluntary exercise
• ISSN: 2211-1247; 2211-1247
• DOI: 10.1016/j.celrep.2024.114697

Physical activity reduces cancer-associated mortality through multiple mechanisms, including tumor immune microenvironment (TIME) reprogramming. However, whether and how physiological interventions promote anti-tumor immunity remain elusive. Here, we report that clinically relevant voluntary exercise promotes muscle-derived extracellular vesicle (EV)-associated miR-29a-3p for tumor extracellular matrix (ECM) inhibition in patients and mouse models, thereby permitting immune cell infiltration and immunotherapy. Mechanistically, an unbiased screening identifies EV-associated miR-29a-3p in response to leisure-time physical activity or voluntary exercise. MiR-29a-3p-containing EVs accumulate in tumors and downregulate collagen composition by targeting COL1A1. Gain- and loss-of-function experiments and cytometry by time of flight (CyTOF) demonstrate that myocyte-secreted miR-29a-3p promotes anti-tumor immunity. Combining immunotherapy with voluntary exercise or miR-29a-3p further enhances anti-tumor efficacy. Clinically, miR-29a-3p correlates with reduced ECM, increased T cell infiltration, and response to immunotherapy. Our work reveals the predictive value of miR-29a-3p for immunotherapy, provides mechanistic insights into exercise-induced anti-cancer immunity, and highlights the potential of voluntary exercise in sensitizing immunotherapy. [Display omitted] •Voluntary exercise increases miR-29a-3p in muscle-derived EVs in patients with cancer•MiR-29a-3p-containing EVs accumulate in tumors and inhibit collagen components•Exercise or collagen inhibition leads to tumor inflammation and sensitizes immunotherapy Luo et al. report that voluntary exercise sensitizes immunotherapy in mouse models and patients with cancer by releasing muscle-derived miR-29a-3p-containing EVs. These EVs accumulate in tumor tissue and inhibit collagen components, leading to an inflammatory tumor immune microenvironment and sensitizing immunotherapy.