Ganglioside Composition Distinguishes Anaplastic Ganglioglioma Tumor Tissue from Peritumoral Brain Tissue: Complementary Mass Spectrometry and Thin-Layer Chromatography Evidence

Gangliosides serve as antitumor therapy targets and aberrations in their composition strongly correlate with tumor growth and invasiveness. Anaplastic ganglioglioma is a rare, poorly characterized, malignant neuronal–glial tumor type. We present the first comparative characterization of ganglioside...

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Bibliographic Details
Published in:International journal of molecular sciences 2021-08, Vol.22 (16), p.8844
Main Authors: Fabris, Dragana, Karmelić, Ivana, Muharemović, Hasan, Sajko, Tomislav, Jurilj, Mia, Potočki, Slavica, Novak, Ruđer, Vukelić, Željka
Format: Article
Language:English
Subjects:
Apoptosis
Brain cancer
Brain tumors
Ceramide
ceramides
Chromatography
Composition
Fatty acids
ganglioglioma
Gangliosides
Glycan
Invasiveness
Mass spectrometry
Mass spectroscopy
Metabolism
Metabolites
Neuronal-glial interactions
Residues
Spectroscopy
sphingolipids
Thin layer chromatography
Tumors
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Summary:Gangliosides serve as antitumor therapy targets and aberrations in their composition strongly correlate with tumor growth and invasiveness. Anaplastic ganglioglioma is a rare, poorly characterized, malignant neuronal–glial tumor type. We present the first comparative characterization of ganglioside composition in anaplastic ganglioglioma vs. peritumoral and healthy brain tissues by combining mass spectrometry and thin-layer chromatography. Anaplastic ganglioglioma ganglioside composition was highly distinguishable from both peritumoral and healthy tissue despite having five to six times lower total content. Ten out of twelve MS-identified ganglioside classes, defined by unique glycan residues, were represented by a large number and considerable abundance of individual species with different fatty acid residues (C16–C24) in ceramide portions. The major structurally identified class was tumor-associated GD3 (>50%) with 11 species; GD3 (d18:1/24:0) being the most abundant. The dominant sphingoid base residue in ganglioside ceramides was sphingosine (d18:1), followed by eicosasphingosine (d20:1). The peritumoral tissue ganglioside composition was estimated as normal. Specific ganglioside composition and large variability of ganglioside ceramide structures determined in anaplastic ganglioglioma demonstrate realistic ganglioside expression patterns and correspond to the profile of high-grade malignancy brain tumors.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22168844