Evaluation of the application of enemas containing sucralfate in tissue content of neutral and acid mucins in experimental model of diversion colitis

PURPOSE:To evaluate the effects of sucralfate on tissue content of neutral and acids mucins in rats with diversion colitis.METHODS: Thirty-six rats were submitted to a proximal right colostomy and a distal mucous fistula. They were divided into two groups according to sacrifice to be performed two o...

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Published in:Acta cirúrgica brasileira 2014-09, Vol.29 (9), p.544-552
Main Authors: Chaim, Felipe Mendonça, Sato, Daniela Tiemi, Rodrigues, Murilo Rocha, Dias, Alice Moreira, Silveira Júnior, Paulo Pedroso, Pereira, José Aires, Martinez, Carlos Augusto Real
Format: Article
Language:English
Subjects:
Colitis
Colostomy
Fatty Acids, Volatile
Image Processing, Computer-Assisted
Mucins
Rats
Sucralfate
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Summary:PURPOSE:To evaluate the effects of sucralfate on tissue content of neutral and acids mucins in rats with diversion colitis.METHODS: Thirty-six rats were submitted to a proximal right colostomy and a distal mucous fistula. They were divided into two groups according to sacrifice to be performed two or four weeks after intervention. Each group was divided into three subgroups according daily application of enemas containing saline, sucralfate at 1.0 g/kg/day or 2.0 g/kg/day. Colitis was diagnosed by histological analysis and neutral and acid mucins by Periodic Acid Schiff and Alcian Blue techniques, respectively. The contents of mucins were quantified by computer-assisted image analysis. Student's t paired and ANOVA test were used to compare the contents of both types of mucins among groups, and to verify the variance with time, establishing level of signification of 5% for both (p
ISSN:0102-8650
0102-8650
DOI:10.1590/S0102-8650201400150001