Evaluation of the application of enemas containing sucralfate in tissue content of neutral and acid mucins in experimental model of diversion colitis

PURPOSE:To evaluate the effects of sucralfate on tissue content of neutral and acids mucins in rats with diversion colitis.METHODS: Thirty-six rats were submitted to a proximal right colostomy and a distal mucous fistula. They were divided into two groups according to sacrifice to be performed two o...

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Published in:Acta cirúrgica brasileira 2014-09, Vol.29 (9), p.544-552
Main Authors: Chaim, Felipe Mendonça, Sato, Daniela Tiemi, Rodrigues, Murilo Rocha, Dias, Alice Moreira, Silveira Júnior, Paulo Pedroso, Pereira, José Aires, Martinez, Carlos Augusto Real
Format: Article
Language:English
Subjects:
Colitis
Colostomy
Fatty Acids, Volatile
Image Processing, Computer-Assisted
Mucins
Rats
Sucralfate
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cited_by cdi_FETCH-LOGICAL-c431t-ccd92185ef5d9b7a73fcf0a796b612188a5f5ccfff2c285d7ec79c8d29ea422d3
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container_title Acta cirúrgica brasileira
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creator Chaim, Felipe Mendonça
Sato, Daniela Tiemi
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Martinez, Carlos Augusto Real
description PURPOSE:To evaluate the effects of sucralfate on tissue content of neutral and acids mucins in rats with diversion colitis.METHODS: Thirty-six rats were submitted to a proximal right colostomy and a distal mucous fistula. They were divided into two groups according to sacrifice to be performed two or four weeks after intervention. Each group was divided into three subgroups according daily application of enemas containing saline, sucralfate at 1.0 g/kg/day or 2.0 g/kg/day. Colitis was diagnosed by histological analysis and neutral and acid mucins by Periodic Acid Schiff and Alcian Blue techniques, respectively. The contents of mucins were quantified by computer-assisted image analysis. Student's t paired and ANOVA test were used to compare the contents of both types of mucins among groups, and to verify the variance with time, establishing level of signification of 5% for both (p
doi_str_mv 10.1590/S0102-8650201400150001
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The content of neutral mucins does not change with the time or concentration of sucralfate used, while acid mucins increases with concentration and time of intervention.CONCLUSIONS:Sucralfate enemas improve the inflammatory process and increase the tissue content of neutral and acid mucins in colon without fecal stream.</description><subject>Colitis</subject><subject>Colostomy</subject><subject>Fatty Acids, Volatile</subject><subject>Image Processing, Computer-Assisted</subject><subject>Mucins</subject><subject>Rats</subject><subject>Sucralfate</subject><issn>0102-8650</issn><issn>0102-8650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNpdkd1KxDAQhYsouP68guQFVpO0adpLkfUHBC_U6zI7mWikmyxNuuiD-L6mu7KIN0k458w3gVMUF4JfCtXyq2cuuJw3teKSi4pzoXg-DorZXj_88z4uTmL8yIGqFuWs-F5soB8hueBZsCy9E4P1une4l8jTCiLD4BM47_wbiyMO0FtIxJxnycU40tYnn6YJT2PKAQbeMEBn2GpE5-MUps81DW6Vg9lfBUP9NGDchoY47cPQuww8K44s9JHOf-_T4vV28XJzP398unu4uX6cY1WKNEc0rRSNIqtMu9SgS4uWg27rZS2y0YCyCtFaK1E2ymhC3WJjZEtQSWnK0-JhxzUBPrp1_hkMX10A122FMLx1MCSHPXUSRMm5Wgqr60pb2apWLpUFTboGLTCz6h0LhxDjQHbPE7ybiuq2RXX_iyp_AMEoigc</recordid><startdate>201409</startdate><enddate>201409</enddate><creator>Chaim, Felipe Mendonça</creator><creator>Sato, Daniela Tiemi</creator><creator>Rodrigues, Murilo Rocha</creator><creator>Dias, Alice Moreira</creator><creator>Silveira Júnior, Paulo Pedroso</creator><creator>Pereira, José Aires</creator><creator>Martinez, Carlos Augusto Real</creator><general>Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia</general><scope>AAYXX</scope><scope>CITATION</scope><scope>DOA</scope></search><sort><creationdate>201409</creationdate><title>Evaluation of the application of enemas containing sucralfate in tissue content of neutral and acid mucins in experimental model of diversion colitis</title><author>Chaim, Felipe Mendonça ; Sato, Daniela Tiemi ; Rodrigues, Murilo Rocha ; Dias, Alice Moreira ; Silveira Júnior, Paulo Pedroso ; Pereira, José Aires ; Martinez, Carlos Augusto Real</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-ccd92185ef5d9b7a73fcf0a796b612188a5f5ccfff2c285d7ec79c8d29ea422d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Colitis</topic><topic>Colostomy</topic><topic>Fatty Acids, Volatile</topic><topic>Image Processing, Computer-Assisted</topic><topic>Mucins</topic><topic>Rats</topic><topic>Sucralfate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chaim, Felipe Mendonça</creatorcontrib><creatorcontrib>Sato, Daniela Tiemi</creatorcontrib><creatorcontrib>Rodrigues, Murilo Rocha</creatorcontrib><creatorcontrib>Dias, Alice Moreira</creatorcontrib><creatorcontrib>Silveira Júnior, Paulo Pedroso</creatorcontrib><creatorcontrib>Pereira, José Aires</creatorcontrib><creatorcontrib>Martinez, Carlos Augusto Real</creatorcontrib><collection>CrossRef</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Acta cirúrgica brasileira</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chaim, Felipe Mendonça</au><au>Sato, Daniela Tiemi</au><au>Rodrigues, Murilo Rocha</au><au>Dias, Alice Moreira</au><au>Silveira Júnior, Paulo Pedroso</au><au>Pereira, José Aires</au><au>Martinez, Carlos Augusto Real</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of the application of enemas containing sucralfate in tissue content of neutral and acid mucins in experimental model of diversion colitis</atitle><jtitle>Acta cirúrgica brasileira</jtitle><date>2014-09</date><risdate>2014</risdate><volume>29</volume><issue>9</issue><spage>544</spage><epage>552</epage><pages>544-552</pages><issn>0102-8650</issn><eissn>0102-8650</eissn><abstract>PURPOSE:To evaluate the effects of sucralfate on tissue content of neutral and acids mucins in rats with diversion colitis.METHODS: Thirty-six rats were submitted to a proximal right colostomy and a distal mucous fistula. 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subjects Colitis
Colostomy
Fatty Acids, Volatile
Image Processing, Computer-Assisted
Mucins
Rats
Sucralfate
title Evaluation of the application of enemas containing sucralfate in tissue content of neutral and acid mucins in experimental model of diversion colitis
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