Escherichia coli SPFH Membrane Microdomain Proteins HflKC Contribute to Aminoglycoside and Oxidative Stress Tolerance

• Published in: Microbiology spectrum 2023-08, Vol.11 (4), p.e0176723-e0176723
• Main Authors: Wessel, Aimee K, Yoshii, Yutaka, Reder, Alexander, Boudjemaa, Rym, Szczesna, Magdalena, Betton, Jean-Michel, Bernal-Bayard, Joaquin, Beloin, Christophe, Lopez, Daniel, Völker, Uwe, Ghigo, Jean-Marc
• Format: Article
• Language: English
• Subjects: Bacteriology; Escherichia coli; flotillin; Life Sciences; lipid raft; membrane microdomains; Microbiology and Parasitology; SPFH proteins; stress tolerance
• ISSN: 2165-0497; 2165-0497
• DOI: 10.1128/spectrum.01767-23

Many eukaryotic membrane-dependent functions are often spatially and temporally regulated by membrane microdomains (FMMs), also known as lipid rafts. These domains are enriched in polyisoprenoid lipids and scaffolding proteins belonging to the tomatin, rohibitin, lotillin, and flK/C (SPFH) protein superfamily that was also identified in Gram-positive bacteria. In contrast, little is still known about FMMs in Gram-negative bacteria. In Escherichia coli K-12, 4 SPFH proteins, YqiK, QmcA, HflK, and HflC, were shown to localize in discrete polar or lateral inner membrane locations, raising the possibility that E. coli SPFH proteins could contribute to the assembly of inner membrane FMMs and the regulation of cellular processes. Here, we studied the determinant of the localization of QmcA and HflC and showed that FMM-associated cardiolipin lipid biosynthesis is required for their native localization pattern. Using Biolog phenotypic arrays, we showed that a mutant lacking all SPFH genes displayed increased sensitivity to aminoglycosides and oxidative stress that is due to the absence of HflKC. Our study therefore provides further insights into the contribution of SPFH proteins to stress tolerance in E. coli. Eukaryotic cells often segregate physiological processes in cholesterol-rich functional membrane microdomains. These domains are also called lipid rafts and contain proteins of the tomatin, rohibitin, lotillin, and flK/C (SPFH) superfamily, which are also present in prokaryotes but have been mostly studied in Gram-positive bacteria. Here, we showed that the cell localization of the SPFH proteins QmcA and HflKC in the Gram-negative bacterium E. coli is altered in the absence of cardiolipin lipid synthesis. This suggests that cardiolipins contribute to E. coli membrane microdomain assembly. Using a broad phenotypic analysis, we also showed that HflKC contribute to E. coli tolerance to aminoglycosides and oxidative stress. Our study, therefore, provides new insights into the cellular processes associated with SPFH proteins in E. coli.