Staphylococcus aureus Small Colony Variants (SCVs): News From a Chronic Prosthetic Joint Infection

Small colony variants (SCV) of have been reported as implicated in chronic infections. Here, we investigated the genomic and transcriptomic changes involved in the evolution from a wild-type to a SCV from in a patient with prosthetic joint infection relapse. The SCV presented a stable phenotype with...

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Bibliographic Details
Published in:Frontiers in cellular and infection microbiology 2019-10, Vol.9, p.363-363
Main Authors: Loss, Guilherme, Simões, Patricia Martins, Valour, Florent, Cortês, Marina Farrel, Gonzaga, Luiz, Bergot, Marine, Trouillet-Assant, Sophie, Josse, Jêrome, Diot, Alan, Ricci, Emiliano, Vasconcelos, Ana Tereza, Laurent, Frédéric
Format: Article
Language:English
Subjects:
Cellular and Infection Microbiology
chronic infection
Human health and pathology
Infectious diseases
Life Sciences
prosthetic joint infection
rifampicin resistance
small colony variant
Staphylococcus aureus
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Summary:Small colony variants (SCV) of have been reported as implicated in chronic infections. Here, we investigated the genomic and transcriptomic changes involved in the evolution from a wild-type to a SCV from in a patient with prosthetic joint infection relapse. The SCV presented a stable phenotype with no classical auxotrophy and the emergence of rifampicin resistance. Whole Genome Sequencing (WGS) analysis showed only the loss of a 42.5 kb phage and 3 deletions, among which one targeting the gene, known to be the target of rifampicin and to be associated to SCV formation in the context of a constitutively active stringent response. Transcriptomic analysis highlighted a specific signature in the SCV strain including a complex, multi-level strategy of survival and adaptation to chronicity within the host including a protection from the inflammatory response, an evasion of the immune response, a constitutively activated stringent response and a scavenging of iron sources.
ISSN:2235-2988
2235-2988
DOI:10.3389/fcimb.2019.00363