Gamma irradiation green synthesis of (polyacrylamide/chitosan/silver nanoparticles) hydrogel nanocomposites and their using as antifungal against Candida albicans and anti-cancer modulator

• Published in: Scientific reports 2024-10, Vol.14 (1), p.25758-16, Article 25758
• Main Authors: Khalil, Salwa A., Awadallah-F, Ahmed, Khaffaga, Mervat R., Fathy, Rasha Mohammad, Kodous, Ahmad S.
• Format: Article
• Language: English
• Subjects: 639/301; 639/301/1023; 639/301/1023/1025; Acrylamide; Acrylic Resins - chemistry; Acrylic Resins - pharmacology; Anti-cancer; Antifungal activity; Antifungal Agents - chemical synthesis; Antifungal Agents - chemistry; Antifungal Agents - pharmacology; Antimicrobial; Antineoplastic Agents - chemical synthesis; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Antioxidants; Antitumor activity; Apoptosis; Bcl-2 protein; Cancer; Candida albicans - drug effects; Cervical cancer; Chitosan; Chitosan - chemistry; Chitosan - pharmacology; Cytotoxicity; Down-regulation; Fourier analysis; Fourier transforms; Gamma irradiation; Gamma Rays; Gelatinase A; Gene expression; Gravimetric analysis; Green Chemistry Technology - methods; HeLa Cells; Humanities and Social Sciences; Humans; Hydrogel; Hydrogels; Hydrogels - chemistry; Hydrogels - pharmacology; Infrared spectroscopy; Irradiation; Metal Nanoparticles - chemistry; Microbial Sensitivity Tests; Microscopy; multidisciplinary; Nanocomposite; Nanocomposites; Nanocomposites - chemistry; Nanoparticles; p53 Protein; Polyacrylamide; Scanning electron microscopy; Science; Science (multidisciplinary); Silver; Silver - chemistry; Silver - pharmacology; Spectroscopy, Fourier Transform Infrared; Transmission electron microscopy; X-ray diffraction; γ Radiation
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-74027-x

Silver nanoparticles-loaded hydrogel nanocomposites are exploited for medicinal and pharmaceutical applications. Hydrogel nanocomposites were prepared from acrylamide (Am), chitosan (CS) and AgNO 3 utilizing gamma rays. Diverse variables were applied in preparation of silver nanoparticles-laoded hydrogel nanocomposites of (PAm/CS)-AgNPs such as influence of radiation dose and influnece of CS concentration. Diverse techniques were utilized to characterize hydrogel nanocomposites; Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), X-ray diffraction (XRD), Transmission electron microscopy (TEM), energy dispersive X-ray (EDX) and scanning electron microscopy (SEM). Results confirmed formation of silver nanoparticles-loaded hydrogel nanocomposites of (PAm/CS)-AgNPs. Antifungal activity of (PAm/CS)-AgNPs hydrogel nanocomposites on viability of C. albicans was esitmated. Results displayed the efficient microbial inhibition activity of treatment against C. albicans compared to control. Furthermore, (PAm/CS)-AgNPs hydrogel nanocomposite against cervical cancer HeLa cell line was investigated. Cytotoxicity of (PAm/CS)-AgNPs hydrogel nanocomposites on prior cancer cell line empolyed to prohibition of cell growth assesssed by MTT test. HeLa cancer cell is treated by (PAm/CS)-AgNPs for 48 h exposed a potential apoptotic activity by noticeable up-regulation of p53 gene expression. Moreover, anticancer activity was investigated by down-regulation of platelet-based growth variable receptor beta (PDGFR-β), Bcl2, Cathepsine, and MMP-2 gene expression. antioxidant activity was investigated and results showed antioxidant activity of (PAm/CS) hydrogel and (PAm/CS)-AgNPs hydrogel nanocomposite are 87.8% and 62.9%, respectively.