Galectin-3 and HBME-1 Expression on agarose cell blocks from fine-needle aspirates of follicular cell-derived thyroid tumors

Aim: To test the expression of galectin-3 (gal-3) and Hector Battifora mesothelial antigen-1 (HBME-1) on agarose cell blocks (CBs) of fine-needle aspirates aiming to determine their diagnostic value in thyroid follicle cell-derived tumors. Materials and Methods: Forty patients with thyroid nodule we...

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Bibliographic Details
Published in:Journal of cytology 2018-01, Vol.35 (1), p.27-32
Main Authors: Abu-Sinna, E, Hasan, M, El-Deftar, M, Amer, S, Abdelsalam, L, Nakhla, J
Format: Article
Language:English
Subjects:
Adenoma
Agarose cell block
Antibodies
Antigens
Biopsy
Cell adhesion & migration
Cellular biology
Diagnosis
fine-needle aspirate
Galectin-3
HBME-1
Medical research
Medicine
Original
Papillary thyroid carcinoma
Pathology
Proteins
Stains & staining
Studies
Systematic review
Thyroid
Thyroid cancer
Thyroid diseases
Thyroid gland
thyroid tumors
Tumors
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Summary:Aim: To test the expression of galectin-3 (gal-3) and Hector Battifora mesothelial antigen-1 (HBME-1) on agarose cell blocks (CBs) of fine-needle aspirates aiming to determine their diagnostic value in thyroid follicle cell-derived tumors. Materials and Methods: Forty patients with thyroid nodule were enrolled. Fine-needle aspiration biopsy was done and processed to produce smears and CBs. Immune staining was done on CBs using antibodies to gal-3 and HBME-1. Diagnostic value of tests was determined in comparison to pathology. Results: Current study included 17 papillary thyroid carcinoma (PTC), 15 follicular adenoma, and 8 follicular thyroid carcinoma (FTC). In PTC diagnosis, co-expression of gal-3/HBME-1 had a sensitivity of 94.1%, specificity of 73.3%, positive predictive value (PPV) of 80%, negative predictive value (NPV) 91.7%, and accuracy of 84.4%. In FTC diagnosis, combined gal-3/HBME-1 expression had a sensitivity of 75%, specificity of 78.6%, PPV of 50%, NPV of 91.7%, and accuracy of 77.8%. Co-expression of gal-3/HBME-1 raised the sensitivity of detection of differentiated thyroid carcinomas from 40% with cytomorphology to 92% and accuracy from 62.5% to 85%. Conclusion: The combined immunocytochemical expression of gal-3 and HBME-1 utilizing fine-needle aspirates can improve the sensitivity of detection and diagnostic accuracy of differentiated follicular cell-derived thyroid carcinomas.
ISSN:0970-9371
0974-5165
DOI:10.4103/JOC.JOC_67_17