Differential expression profiles of microRNAs in musk gland of unmated and mated forest musk deer ( Moschus berezovskii )

The formation of musk is a complex biophysical and biochemical process that change with the rut of male forest musk deer. We have reported that the mating status of male forest musk deer might result to the variations of chemical composition and microbiota of musk and its yields. Critical roles for...

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Published in:PeerJ (San Francisco, CA) CA), 2021-12, Vol.9, p.e12710-e12710, Article e12710
Main Authors: Jie, Hang, Xu, Zhongxian, Gao, Jian, Li, Feng, Chen, Yinglian, Zeng, Dejun, Zhao, Guijun, Li, Diyan
Format: Article
Language:English
Subjects:
Animal populations
Biochemistry
Cellular proteins
Circulatory system
Earthquakes
Exocytosis
Expression profile
Forests
Gene expression
Genetics
Glands
Hormones
Males
Mating status
Microbiota
Microbiota (Symbiotic organisms)
MicroRNA
MicroRNAs
miRNA
Molecular Biology
Monosaccharides
Moschidae
Moschus berezovskii
Musk deer
Musk gland
Nucleotide sequence
Software
Transcriptomes
Transfer RNA
Zoology
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Summary:The formation of musk is a complex biophysical and biochemical process that change with the rut of male forest musk deer. We have reported that the mating status of male forest musk deer might result to the variations of chemical composition and microbiota of musk and its yields. Critical roles for microRNAs (miRNAs) of multi-tissues were profiled in our previous study; however, the role for miRNAs of the musk gland remains unclear in this species. In this study, we used Illumina deep sequencing technology to sequence the small RNA transcriptome of unmated male (UM) and mated male (UM) of Chinese forest musk deer. We identified 1,652 known miRNAs and 45 novel miRNAs, of which there were 174 differentially expressed miRNAs between UM and MM. chi-miR-21-5p, ipu-miR-99b and bta-miR-26a were up-regulated in UM among the 10 most differentially expressed miRNAs. Functional enrichment of the target genes showed that monosaccharide biosynthetic process, protein targeting, cellular protein catabolic process enriched higher in MM. Meanwhile, structural molecule activity, secretion by cell, regulated exocytosis and circulatory system process enriched more in UM, hinting that the formation of musk in UM was mediated by target genes related to exocytosis. The miRNA-mRNA pairs such as miR-21: , miR143: , miR-141/200a: might involve in musk gland development and musk secretion, which need to be verified in future study.
ISSN:2167-8359
2167-8359
DOI:10.7717/peerj.12710