Transposon Insertion Site Sequencing of Providencia stuartii: Essential Genes, Fitness Factors for Catheter-Associated Urinary Tract Infection, and the Impact of Polymicrobial Infection on Fitness Requirements

is a common cause of polymicrobial catheter-associated urinary tract infection (CAUTI), and yet literature describing the molecular mechanisms of its pathogenesis is limited. To identify factors important for colonization during single-species infection and during polymicrobial infection with a comm...

Full description

Saved in:
Bibliographic Details
Published in:mSphere 2020-05, Vol.5 (3)
Main Authors: Johnson, Alexandra O, Forsyth, Valerie, Smith, Sara N, Learman, Brian S, Brauer, Aimee L, White, Ashley N, Zhao, Lili, Wu, Weisheng, Mobley, Harry L T, Armbruster, Chelsie E
Format: Article
Language:English
Subjects:
Animal models
Arginine
Biofilms
Bladder
catheter
Catheterization
Catheters
Cell division
Colonization
D-Alanine
D-Alanine-D-alanine ligase
essential genes
Fitness
Flagella
Genes
Genomes
Host-Microbe Biology
Molecular modelling
Mutants
Pathogenesis
Pathogens
Phenotypes
Plasmids
Proteus mirabilis
Providencia stuartii
Secretion
Species
Tn-Seq
Transposons
Urinary tract
Urinary tract diseases
Urinary tract infections
Urogenital system
Virulence
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:is a common cause of polymicrobial catheter-associated urinary tract infection (CAUTI), and yet literature describing the molecular mechanisms of its pathogenesis is limited. To identify factors important for colonization during single-species infection and during polymicrobial infection with a common cocolonizer, , we created a saturating library of ∼50,000 transposon mutants and conducted transposon insertion site sequencing (Tn-Seq) in a murine model of CAUTI. strain BE2467 carries 4,398 genes, 521 of which were identified as essential for growth in laboratory medium and therefore could not be assessed for contribution to infection. Using an input/output fold change cutoff value of 20 and values of
ISSN:2379-5042
2379-5042
DOI:10.1128/mSphere.00412-20