Physical mapping of a new powdery mildew resistance locus from Thinopyrum ponticum chromosome 4AgS

(Podp.) Barkworth and D.R. Dewey is a decaploid species that has served as an important genetic resource for improving wheat for the better part of a century. The wheat- 4Ag (4D) disomic substitution line Blue 58, which was obtained following the distant hybridization between and common wheat, has b...

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Published in:Frontiers in plant science 2023-02, Vol.14, p.1131205-1131205
Main Authors: Yang, Guotang, Deng, Pingchuan, Ji, Wanquan, Fu, Shulan, Li, Hongwei, Li, Bin, Li, Zhensheng, Zheng, Qi
Format: Article
Language:English
Subjects:
4Ag
cytogenetic analysis
physical mapping
Plant Science
Pm gene
specific marker amplification
Thinopyrum ponticum
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Summary:(Podp.) Barkworth and D.R. Dewey is a decaploid species that has served as an important genetic resource for improving wheat for the better part of a century. The wheat- 4Ag (4D) disomic substitution line Blue 58, which was obtained following the distant hybridization between and common wheat, has been stably resistant to powdery mildew under field conditions for more than 40 years. The transfer of 4Ag into the susceptible wheat cultivar Xiaoyan 81 resulted in powdery mildew resistance, indicating the alien chromosome includes the resistance locus. Irradiated Blue 58 pollen were used for the pollination of the recurrent parent Xiaoyan 81, which led to the development of four stable wheat- 4Ag translocation lines with diverse alien chromosomal segments. The assessment of powdery mildew resistance showed that translocation line L1 was susceptible, but the other three translocation lines (WTT139, WTT146, and WTT323) were highly resistant. The alignment of 81 specific-locus amplified fragments to the genome revealed that 4Ag originated from a group 4 chromosome. The corresponding physical positions of every 4Ag-derived fragment were determined according to a cytogenetic analysis, the amplification of specific markers, and a sequence alignment. Considering the results of the evaluation of disease resistance, the locus was mapped to the 3.79-97.12 Mb region of the short arm of chromosome 4Ag. Because of its durability, this newly identified locus from a group 4 chromosome of may be important for breeding wheat varieties with broad-spectrum disease resistance.
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2023.1131205