Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea)

Heteroxenic and monoxenic trypanosomatids were screened for the presence of actin using a mouse polyclonal antibody produced against the entire sequence of the Trypanosoma cruzi actin gene, encoding a 41.9 kDa protein. Western blot analysis showed that this antibody reacted with a polypeptide of app...

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Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz 2013-08, Vol.108 (5), p.631-636
Main Authors: Souza, Ligia Cristina Kalb, Pinho, Rosana Elisa Gonçalves Gonçalves, Lima, Carla Vanessa de Paula, Fragoso, Stênio Perdigão, Soares, Maurilio José
Format: Article
Language:English
Subjects:
actin
Actins - metabolism
Animals
Blotting, Western
confocal microscopy
Crithidia fasciculata
Electrophoresis, Polyacrylamide Gel
Euglenozoa
Fluorescent Antibody Technique
Herpetomonas samuelpessoai
Leishmania major
Mice
Microscopy, Confocal
PARASITOLOGY
Phytomonas serpens
Protozoan Proteins - metabolism
TROPICAL MEDICINE
Trypanosoma brucei
Trypanosoma cruzi
trypanosomatids
Trypanosomatina - metabolism
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Summary:Heteroxenic and monoxenic trypanosomatids were screened for the presence of actin using a mouse polyclonal antibody produced against the entire sequence of the Trypanosoma cruzi actin gene, encoding a 41.9 kDa protein. Western blot analysis showed that this antibody reacted with a polypeptide of approximately 42 kDa in the whole-cell lysates of parasites targeting mammals (T. cruzi, Trypanosoma brucei and Leishmania major), insects (Angomonas deanei, Crithidia fasciculata, Herpetomonas samuelpessoai and Strigomonas culicis) and plants (Phytomonas serpens). A single polypeptide of approximately 42 kDa was detected in the whole-cell lysates of T. cruzi cultured epimastigotes, metacyclic trypomastigotes and amastigotes at similar protein expression levels. Confocal microscopy showed that actin was expressed throughout the cytoplasm of all the tested trypanosomatids. These data demonstrate that actin expression is widespread in trypanosomatids.
ISSN:0074-0276
1678-8060
1678-8060
0074-0276
DOI:10.1590/0074-0276108052013015