Protective Effects of Polydatin from Grapes and Reynoutria japonica Houtt. on Damaged Macrophages Treated with Acetaminophen

The unregulated use of acetaminophen (APAP), an antipyretic and analgesic drug, harms hepatocytes and kidney cells, leading to liver failure and acute kidney injury. Herein, we investigate whether APAP damages macrophages in the immune system by observing its effects on macrophage proliferation and...

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Bibliographic Details
Published in:Nutrients 2022-05, Vol.14 (10), p.2077
Main Authors: Liu, Can, Wang, Wenyi, Zhang, Kaixin, Liu, Qiudi, Ma, Tongyao, Tan, Li, Ma, Lanqing
Format: Article
Language:English
Subjects:
Acetaminophen
Acetaminophen - pharmacology
Analgesics
Apoptosis
Biological activity
Cell culture
Cell division
Chemical and Drug Induced Liver Injury - metabolism
Cytology
Drug dosages
Enzymes
Flow cytometry
Glucosides
Grapes
Hepatocytes
Immune system
in vitro
Inflammation
Kidneys
Liver
Liver diseases
Macrophages
Macrophages - metabolism
Morphology
Pathogens
polydatin
protective effects
Proteins
Proteomics
Renal failure
Repair
Reynoutria
Stilbenes
Vitis
Wines
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Summary:The unregulated use of acetaminophen (APAP), an antipyretic and analgesic drug, harms hepatocytes and kidney cells, leading to liver failure and acute kidney injury. Herein, we investigate whether APAP damages macrophages in the immune system by observing its effects on macrophage proliferation and apoptosis. Using proteomics, we analyzed the effects of APAP on macrophage protein expression profiles and evaluated whether polydatin, the active ingredient in grapes and wine, can repair the damaged cells. The results showed that APAP alters the morphology and physiological processes of macrophages, inhibits macrophage proliferation, and promotes apoptosis. We observed 528 differentially expressed proteins when 500 µg/mL APAP was administered to the cells. These proteins are involved in biological processes including cell division, apoptosis, and acute phase response. Overall, our findings demonstrate that APAP harms the immune system by damaging macrophages and that polydatin can repair this damage.
ISSN:2072-6643
2072-6643
DOI:10.3390/nu14102077