Recent Advances in CRISPR/Cas9-Mediated Genome Editing in Dictyostelium

In the last 30 years, knockout of target genes via homologous recombination has been widely performed to clarify the physiological functions of proteins in . As of late, CRISPR/Cas9-mediated genome editing has become a versatile tool in various organisms, including , enabling rapid high-fidelity mod...

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Bibliographic Details
Published in:Cells (Basel, Switzerland) Switzerland), 2019-01, Vol.8 (1), p.46
Main Authors: Muramoto, Tetsuya, Iriki, Hoshie, Watanabe, Jun, Kawata, Takefumi
Format: Article
Language:English
Subjects:
Amoebozoa
Cell division
Cloning
CRISPR
CRISPR vector
CRISPR-Associated Protein 9 - genetics
CRISPR-Cas Systems
CRISPR/Cas9
Dictyostelium
Dictyostelium - genetics
Efficiency
Gene Editing - trends
Genes
Genetic Vectors - genetics
genome editing
Genomes
Homologous Recombination
Kinases
Mutagenesis
Mutation
Organisms
Plasmids
Proteins
Review
RNA polymerase
tRNA-based expression
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Summary:In the last 30 years, knockout of target genes via homologous recombination has been widely performed to clarify the physiological functions of proteins in . As of late, CRISPR/Cas9-mediated genome editing has become a versatile tool in various organisms, including , enabling rapid high-fidelity modification of endogenous genes. Here we reviewed recent progress in genome editing in and summarised useful CRISPR vectors that express sgRNA and Cas9, including several microorganisms. Using these vectors, precise genome modifications can be achieved within 2⁻3 weeks, beginning with the design of the target sequence. Finally, we discussed future perspectives on the use of CRISPR/Cas9-mediated genome editing in .
ISSN:2073-4409
2073-4409
DOI:10.3390/cells8010046