The Potential of CD16 on Plasma-Derived Exosomes as a Liquid Biomarker in Head and Neck Cancer

Head and neck squamous cell carcinomas (HNSCC) are highly immune suppressive and aggressive malignancies. As part of the tumor microenvironment, exosomes contribute to this immune suppression. The Fc receptor CD16 is widely expressed on monocytes, neutrophils, and natural killer (NK) cells and is in...

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Bibliographic Details
Published in:International journal of molecular sciences 2020-05, Vol.21 (11), p.3739
Main Authors: Hofmann, Linda, Ludwig, Sonja, Schuler, Patrick J, Hoffmann, Thomas K, Brunner, Cornelia, Theodoraki, Marie-Nicole
Format: Article
Language:English
Subjects:
Biomarkers, Tumor - blood
Biomarkers, Tumor - metabolism
Carcinoma, Squamous Cell - blood
Carcinoma, Squamous Cell - pathology
CD16
Cell Line, Tumor
exosomes
Exosomes - metabolism
Female
Head and Neck Neoplasms - blood
Head and Neck Neoplasms - pathology
HNSCC
Humans
liquid biomarker
Male
Middle Aged
Receptors, IgG - blood
Receptors, IgG - metabolism
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Summary:Head and neck squamous cell carcinomas (HNSCC) are highly immune suppressive and aggressive malignancies. As part of the tumor microenvironment, exosomes contribute to this immune suppression. The Fc receptor CD16 is widely expressed on monocytes, neutrophils, and natural killer (NK) cells and is involved in antibody-dependent cell-mediated cytotoxicity (ADCC). Here, surface levels of CD16 on total exosomes and tumor-derived exosomes (TEX) from plasma of HNSCC patients were analyzed regarding their potential as liquid biomarkers for disease stage. Exosomes were isolated from plasma using mini size exclusion chromatography. TEX were enriched by immune affinity capture with CD44v3 antibodies. On-bead flow cytometry was used to measure CD16 levels on total exosomes and TEX. The results were correlated with clinicopathological parameters. Total exosomes from HNSCC patients had significantly higher CD16 levels compared to TEX. Further, CD16 surface levels of total exosomes, but not TEX, correlated with clinicopathological parameters. Patients with advanced tumor stages T3/4 and Union for International Cancer Control (UICC) stages III/IV had significantly higher CD16 levels on total exosomes compared to patients with early tumor stages T1/2 and UICC stages I/II, respectively. Overall, CD16 positive exosomes have the potential as liquid biomarkers for HNSCC tumor stage and aggressiveness.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms21113739