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Construction of circRNA-miRNA-mRNA ceRNA regulatory network and screening of diagnostic targets for tuberculosis

Tuberculosis (TB) is a chronic infectious disease caused by ( ), which threatens human health and safety all over the world. Hundreds of thousands of people die from TB every year. Timely early diagnosis and treatment of patients is the most important measure to control the source of infection and c...

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Published in:Annals of medicine (Helsinki) 2024-12, Vol.56 (1), p.2416604
Main Authors: Pu, Xinyi, Sheng, Siyu, Fu, Yujuan, Yang, Yue, Xu, Guangyu
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Sheng, Siyu
Fu, Yujuan
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Xu, Guangyu
description Tuberculosis (TB) is a chronic infectious disease caused by ( ), which threatens human health and safety all over the world. Hundreds of thousands of people die from TB every year. Timely early diagnosis and treatment of patients is the most important measure to control the source of infection and curb the epidemic of tuberculosis. The existing diagnostic methods have the disadvantages of poor sensitivity and long culture time. Competitive endogenous RNAs (ceRNAs) can regulate the expression of corresponding target genes by competing for the same microRNA (miRNA) response elements (MREs) as mRNA. Recent studies have found that circRNA has the advantages of long half-life, good stability and tissue specificity, and can be used as a biomarker for predicting, diagnosing and treating various diseases, and is an ideal candidate for biomarkers in body fluid biopsy. In this study, transcriptome sequencing was performed on whole blood samples to screen out TB-related mirna and mRNA differential expression, and to construct the ceRNA regulatory network. Through the analysis of ceRNA regulatory network, it was found that circRNA could competitively bind has-miR-607 and induce down-regulation of has-miR-607, thereby inhibiting the expression of IFNG. The hsa_circ_0000566, hsa_circ_0001844, hsa_circ_0005408, hsa_circ_0007587, hsa_circ_0086710, IFNG and has-miR-607 couble be used as new diagnostic targets for TB. The results of this study not only provide a new perspective for studying the potential role of ceRNA regulatory network in tuberculosis, but also provide a new target and method for the diagnosis of tuberculosis.
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subjects Biomarkers - blood
Biomarkers - metabolism
ceRNA
circRNA
diagnostic target
Female
Gene Regulatory Networks
Humans
Immunology
Male
MicroRNAs - genetics
MicroRNAs - metabolism
miRNA
Mycobacterium tuberculosis - genetics
regulatory network
RNA, Circular - genetics
RNA, Circular - metabolism
RNA, Competitive Endogenous
RNA, Messenger - metabolism
tuberculosis
Tuberculosis - diagnosis
Tuberculosis - genetics
title Construction of circRNA-miRNA-mRNA ceRNA regulatory network and screening of diagnostic targets for tuberculosis
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